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Live imaging of avian embryos

Kulesa, Paul M. and Fraser, Scott E. (2011) Live imaging of avian embryos. In: Imaging in Developmental Biology: a laboratory maniual. Cold Spring Harbor Laboratory Press , Cold Spring Harbor, NY, pp. 85-99. ISBN 978-0-87969-940-6. https://resolver.caltech.edu/CaltechAUTHORS:20160203-160125426

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Abstract

Our understanding of vertebrate development relies on the ability to visualize cell and tissue behaviors. However, imaging a live embryo poses the dual challenges of achieving single-cell resolution and sustaining embryo health. Here, we present the advantages and limitations of live imaging of avian embryos. We highlight innovative modifications to whole-embryo or tissue slice cultures combined with two-photo, confocal, or wide-field microscopy that allow for a greater flexibility to study complex three-dimensional events, such as avian gastrulation and neural crest migration. We discuss advances in cell labeling that include ovo photoactivation of green fluorescent protein and focal electroporation that permit single-cell tracing with higher fidelity and reproducibility. We include exciting advances in avian transgenics research that promise to streamline the process of cell labeling and allow dynamic imaging shortly after egg opening. These advances in live avian embryo imaging, combined with tissue transplantation and accessibility, make avian embryos a powerful model system for studying cellular and molecular mechanisms of morphogenesis, migration, and cell-fate specification.


Item Type:Book Section
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2011 Cold Spring Harbor Press. We thank Professor Dan Kiehart (Duke University) for the idea of the Teflon window, which he has used extensively for Drosophila imaging. PMK thanks the National Institutes of Health (1R01HD057922) and the Stowers Institute for Medical Research for funding. SEF thanks National Institutes of Health (P50HG004071) and the Beckman institute for funding.
Funders:
Funding AgencyGrant Number
NIH1R01HD057922
Stowers Institute for Medical ResearchUNSPECIFIED
NIHP50HG004071
Caltech Beckman InstituteUNSPECIFIED
Record Number:CaltechAUTHORS:20160203-160125426
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160203-160125426
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64213
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:04 Feb 2016 16:23
Last Modified:03 Oct 2019 09:35

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