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Four-dimensional fluorescent imaging of embryonic quail development

Canaria, Christie A. and Lansford, Rusty (2011) Four-dimensional fluorescent imaging of embryonic quail development. In: Imaging in Developmental Biology: a laboratory manual. Cold Spring Harbor Laboratory Press , Cold Spring Harbor, NY, pp. 539-604. ISBN 978-0-87969-940-6. https://resolver.caltech.edu/CaltechAUTHORS:20160203-162840913

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Abstract

Traditionally, our understanding of developmental biology has been based on the fixation and study of embryonic samples. Detailed microscopic scrutiny of static specimens at varying ages allowed for anatomical assessment of tissue development. The advent of confocal and two-photon excitation (PE) microscopy enables researchers to acquire volumetric images in three dimensions (x, y, and z) plus time (t). Here, we present techniques for acquisition and analysis of three-dimensional time-lapse data. Both confocal microscopy and 2PE microscopy techniques are used. In addition, data processing for tiled image stitching and time-lapse analysis is discussed. Additionally, the development of a new transgenic Japanese quail has provided an embryonic model system that is more easily accessible than mammalian models and more efficient to breed than the classic avian model, the chicken. Here we also outline the preparation in vitro of these transgenic quail embryos for imaging.


Item Type:Book Section
ORCID:
AuthorORCID
Lansford, Rusty0000-0002-2159-3699
Additional Information:© 2011 Cold Spring Harbor Laboratory Press.
Record Number:CaltechAUTHORS:20160203-162840913
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160203-162840913
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64215
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:04 Feb 2016 16:36
Last Modified:03 Oct 2019 09:35

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