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Embryonic Explant and Slice Preparations for Studies of Cell Migration and Axon Guidance

Krull, Catherine E. and Kulesa, Paul M. (1997) Embryonic Explant and Slice Preparations for Studies of Cell Migration and Axon Guidance. In: Cellular and Molecular Procedures in Developmental Biology. Current Topics in Developmental Biology. No.36. Academic Press , San Diego, CA, pp. 145-159. ISBN 978-0-12-153136-2. https://resolver.caltech.edu/CaltechAUTHORS:20160204-121557404

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Abstract

This chapter describes methods for preparing explants and slice preparations of the embryonic chick and mouse. In addition, it describes methods for culturing explants of embryonic mouse tissue. Each culture system possesses distinct advantages and should be adaptable to many studies of developmental processes that occur over a wide spatiotemporal range. These preparations maintain several features common to the intact embryo, including tissue structural integrity and the correct distribution of various molecular constituents for at least 2-3 days. Of critical importance, the explants and slices retain their tissue architecture for 1-3 days in culture and possess molecular characteristics typical of intact embryos. Furthermore, these types of preparations allow one to disrupt the embryonic environment through the addition of various blocking reagents and observe the subsequent effects of these manipulations via static images and using time-lapse videomicroscopy. These types of paradigms should continue to offer a rich cellular and molecular arena in which to study early developmental events, including cell migration and process outgrowth.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0070-2153(08)60500-1DOIArticle
http://www.sciencedirect.com/science/article/pii/S0070215308605001PublisherArticle
Additional Information:© 1998 Academic Press.
Series Name:Current Topics in Developmental Biology
Issue or Number:36
Record Number:CaltechAUTHORS:20160204-121557404
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160204-121557404
Official Citation:Catherine E. Krull, Paul M. Kulesa, 8 Embryonic Explant and Slice Preparations for Studies of Cell Migration and Axon Guidance, In: Flora de Pablo, Alberto Ferrús and Claudio D. Stern, Editor(s), Current Topics in Developmental Biology, Academic Press, 1997, Volume 36, Pages 145-159, ISSN 0070-2153, ISBN 9780121531362, http://dx.doi.org/10.1016/S0070-2153(08)60500-1. (http://www.sciencedirect.com/science/article/pii/S0070215308605001)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64228
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:04 Feb 2016 20:48
Last Modified:03 Oct 2019 09:35

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