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Direct Electrochemistry of Endonuclease III in the Presence and Absence of DNA

Gorodetsky, Alon A. and Boal, Amie K. and Barton, Jacqueline K. (2006) Direct Electrochemistry of Endonuclease III in the Presence and Absence of DNA. Journal of the American Chemical Society, 128 (37). pp. 12082-12083. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160209-124711381

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Abstract

The electrochemistry of the base excision repair enzyme Endonuclease III (Endo III) in the presence and absence of DNA has been examined on highly oriented pyrolytic graphite (HOPG). At the surface modified with pyrenated DNA, a reversible signal is observed at 20 mV versus NHE for the [4Fe−4S]^(3+/2+) couple of Endo III, similar to Au. Without DNA modification, oxidative and reductive signals for the [4Fe−4S] cluster of Endo III are found on bare HOPG, allowing a direct comparison between DNA-bound and free redox potentials. These data indicate a shift of approximately −200 mV in the 3+/2+ couple upon binding of Endo III to DNA. This potential shift reflects a difference in affinity for DNA of more than 3 orders of magnitude between the oxidized 3+ and reduced 2+ protein and provides quantitative support for our model utilizing DNA-mediated charge transport to redistribute base excision repair enzymes in the vicinity of damaged DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja064784dDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja064784dPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja064784dPublisherSupporting Information
ORCID:
AuthorORCID
Gorodetsky, Alon A.0000-0002-3811-552X
Boal, Amie K.0000-0002-1234-8472
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:Copyright © 2006 American Chemical Society. Received July 5, 2006. Publication Date (Web): August 29, 2006. We are grateful to the NIH (Grant GM61077) for their financial support and the Parsons Foundation for a fellowship to A.K.B.
Funders:
Funding AgencyGrant Number
NIHGM61077
Ralph M. Parsons FoundationUNSPECIFIED
Issue or Number:37
Record Number:CaltechAUTHORS:20160209-124711381
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160209-124711381
Official Citation:Direct Electrochemistry of Endonuclease III in the Presence and Absence of DNA Alon A. Gorodetsky, Amie K. Boal, and Jacqueline K. Barton Journal of the American Chemical Society 2006 128 (37), 12082-12083 DOI: 10.1021/ja064784d
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64340
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 Feb 2016 21:31
Last Modified:03 Oct 2019 09:36

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