Rajski, Scott R. and Barton, Jacqueline K. (2001) How Different DNA-Binding Proteins Affect Long-Range Oxidative Damage to DNA. Biochemistry, 40 (18). pp. 5556-5564. ISSN 0006-2960. doi:10.1021/bi002684t. https://resolver.caltech.edu/CaltechAUTHORS:20160209-152857175
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160209-152857175
Abstract
Here the effect on DNA-mediated charge transport of binding by a variety of proteins is examined. DNA assemblies were constructed that contain a tethered rhodium intercalator, as photooxidant, as well as two 5‘-GG-3‘ sites flanking the DNA-binding site for the different proteins. By monitoring the ratio of oxidative damage promoted at the guanine doublet situated distal to the protein-binding site versus that at the proximal site as a function of protein binding, the effects of binding the proteins on DNA-mediated charge transport were determined. Proteins examined included both the wild-type and mutant methyltransferase, M.HhaI, which are base-flipping enzymes, the restriction endonuclease R.PvuII, a TATA-binding protein, which kinks the DNA, and the transcription factor Antennapedia homeodomain protein, which binds DNA through a helix−turn−helix motif. In general, it was observed that yields of long-range oxidative damage correlate with protein-dependent alterations in DNA base stacking. Interactions that disturb the DNA π-stack inhibit DNA charge transport. Alternatively, interactions that promote no helix distortion but, as a result of tight packing, may rigidify the π-stack, serve instead to enhance the ability of the DNA base pairs to serve as a conduit for charge transport. Thus, protein binding to DNA modulates long-range charge transport both negatively and positively, depending upon the specific protein/DNA interactions in play. Long-range DNA charge transport and this modulation by protein binding may be important to consider physiologically.
Item Type: | Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| |||||||||
ORCID: |
| |||||||||
Additional Information: | © 2001 American Chemical Society. Received November 22, 2000; Revised Manuscript Received March 1, 2001. Publication Date (Web): April 12, 2001. Supported by NIH Grant GM49216. We thank our close co-workers Sanjay Kumar and Richard J. Roberts of New England Biolabs (Beverly, MA) for samples of wild-type and M.HhaI Q237W. We also thank Professor Carl S. Parker (Caltech) for the generous gift of ANTP-binding domain peptide and Professors John Horton and Xiaodong Cheng of Emory University for samples of R.PvuII. | |||||||||
Funders: |
| |||||||||
Issue or Number: | 18 | |||||||||
DOI: | 10.1021/bi002684t | |||||||||
Record Number: | CaltechAUTHORS:20160209-152857175 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160209-152857175 | |||||||||
Official Citation: | How Different DNA-Binding Proteins Affect Long-Range Oxidative Damage to DNA Scott R. Rajski and Jacqueline K. Barton Biochemistry 2001 40 (18), 5556-5564 DOI: 10.1021/bi002684t | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 64349 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | George Porter | |||||||||
Deposited On: | 09 Feb 2016 23:38 | |||||||||
Last Modified: | 10 Nov 2021 23:29 |
Repository Staff Only: item control page