CaltechAUTHORS
  A Caltech Library Service

Long-Range Oxidative Damage in DNA/RNA Duplexes

Odom, Duncan T. and Barton, Jacqueline K. (2001) Long-Range Oxidative Damage in DNA/RNA Duplexes. Biochemistry, 40 (30). pp. 8727-8737. ISSN 0006-2960. https://resolver.caltech.edu/CaltechAUTHORS:20160209-160654855

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160209-160654855

Abstract

Oxidative damage as a result of DNA-mediated long-range charge transport occurs readily and at high yield in duplex DNA, and it is of interest whether similar damage can occur in duplex oligonucleotides that include both ribo- and deoxyribonucleotides. Assemblies containing RNA and mixed RNA·DNA strands were constructed containing tethered ethidium as a photooxidant. In photooxidation experiments, long-range oxidative damage to the ribose-containing strand of the oligonucleotide duplexes was examined. Hole injection by photoexcited ethidium followed by radical migration to oxidatively susceptible guanines afforded significant damage on ribose-containing strands at long range (∼35 Å). This damage does not differ substantially in yield and location from that found in B-DNA duplexes. No oxidative damage was found upon photooxidation of DNA/RNA duplexes containing tethered metallointercalator, despite the ability of the rhodium complex to promote oxidative damage at a distance in DNA duplexes. This result is attributed to the poor coupling of the rhodium complex into the A-like RNA/DNA duplex. The ability for long-range charge transport to occur in double-stranded nucleic acids of different comformations is considered in light of modeling studies that show interstrand base−base overlap between the opposing, complementary strands that make up RNA/DNA hybrid duplexes. Thus, the possibility of long-range radical migration to effect oxidative damage or signaling may be considered also in the context of transcriptional events.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi0102961DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi0102961PublisherArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2001 American Chemical Society. Received February 12, 2001; Revised Manuscript Received May 31, 2001. Publication Date (Web): July 6, 2001. D.T.O. thanks the Parson's Foundation for a predoctoral fellowship. Supported by NIH Grant GM49216.
Funders:
Funding AgencyGrant Number
Ralph M. Parsons FoundationUNSPECIFIED
NIHGM49216
Issue or Number:30
Record Number:CaltechAUTHORS:20160209-160654855
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160209-160654855
Official Citation:Long-Range Oxidative Damage in DNA/RNA Duplexes Duncan T. Odom and Jacqueline K. Barton Biochemistry 2001 40 (30), 8727-8737 DOI: 10.1021/bi0102961
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64353
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Feb 2016 16:09
Last Modified:03 Oct 2019 09:37

Repository Staff Only: item control page