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DNA Hydrolysis and Oxidative Cleavage by Metal-Binding Peptides Tethered to Rhodium Intercalators

Copeland, Kimberly D. and Fitzsimons, Marilena P. and Houser, Robert P. and Barton, Jacqueline K. (2002) DNA Hydrolysis and Oxidative Cleavage by Metal-Binding Peptides Tethered to Rhodium Intercalators. Biochemistry, 41 (1). pp. 343-356. ISSN 0006-2960. doi:10.1021/bi011793k.

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With the goal of developing artificial nucleases for DNA hydrolysis, metal-coordinating peptides have been tethered to a DNA-intercalating rhodium complex to deliver metal ions to the sugar−phosphate backbone. The intercalator, [Rh(phi)_2bpy‘]Cl_3 [phi = 9,10-phenanthrenequinone diimine; bpy‘ = 4-(butyric acid)-4‘-methyl-2,2‘-bipyridine], provides DNA binding affinity, and a metal-binding peptide contributes reactivity. This strategy for DNA hydrolysis is a general one, and zinc(II)-promoted cleavage has been demonstrated for two widely different tethered metallopeptides. An intercalator coupled with a de novo-designed α helix containing two histidine residues has been demonstrated to cleave both supercoiled plasmid and linear DNA substrates. Mutation of this peptide confirms that the two histidine residues are essential for Zn^(2+) binding and cleavage. Zinc(II)-promoted cleavage of supercoiled plasmid has also been demonstrated with an intercalator−peptide conjugate containing acidic residues and modeled after the active site of the BamHI endonuclease. Other redox-active metals, such as copper, have been delivered to DNA with our intercalator−peptide conjugates to effect oxidative chemistry. Copper cleavage experiments and photocleavage experiments with [Rh(phi)_2bpy‘]^(3+) complement the hydrolysis studies and provide structural information about the interactions between the tethered metallopeptides and DNA. Variation of the rhodium intercalator was also explored, but with a mismatch-specific intercalator, no site-specific hydrolysis was found. These experiments, in which the peptide, the metal cation, and the intercalator components of the conjugate are each varied, illustrate some of the issues involved in creating an artificial nuclease with DNA intercalators and metallopeptides.

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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2002 American Chemical Society. Received September 14, 2001; Revised Manuscript Received November 2, 2001. Publication Date (Web): December 11, 2001. We are grateful to Dr. Ulrike Riese for synthesis of the bpy‘ ‘ ligand and to the Biopolymer Synthesis Facility at the California Institute of Technology for preparation of peptides. This work was supported by the NIH (Grant GM33309). The NIH also provided NRSA predoctoral (K.D.C.) and postdoctoral (R.P.H.) fellowships, and NSERC provided a predoctoral fellowship (M.P.F.).
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NIH Predoctoral FellowshipUNSPECIFIED
NIH Postdoctoral FellowshipUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
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Official Citation:DNA Hydrolysis and Oxidative Cleavage by Metal-Binding Peptides Tethered to Rhodium Intercalators Kimberly D. Copeland, Marilena P. Fitzsimons, Robert P. Houser, and Jacqueline K. Barton Biochemistry 2002 41 (1), 343-356 DOI: 10.1021/bi011793k
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64354
Deposited By: George Porter
Deposited On:10 Feb 2016 16:11
Last Modified:10 Nov 2021 23:29

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