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Studies Using Leukemia Inhibitory Factor (LIF) Knockout Mice and a LIF Adenoviral Vector Demonstrate a Key Anti-Inflammatory Role for This Cytokine in Cutaneous Inflammation

Zhu, Min and Oishi, Kazuhiko and Chul Lee, Sang and Patterson, Paul H. (2001) Studies Using Leukemia Inhibitory Factor (LIF) Knockout Mice and a LIF Adenoviral Vector Demonstrate a Key Anti-Inflammatory Role for This Cytokine in Cutaneous Inflammation. Journal of Immunology, 166 (3). pp. 2049-2054. ISSN 0022-1767. https://resolver.caltech.edu/CaltechAUTHORS:20160210-145408606

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Abstract

Previous work has implicated the cytokine leukemia inhibitory factor (LIF) in cutaneous inflammation, although results have differed as to whether LIF is pro- or anti-inflammatory in this setting. We examined edema, inflammatory cell infiltration, and cytokine responses following CFA injection in the adult mouse footpad. Inflammatory cell infiltration and edema are significantly enhanced when CFA is injected in LIF knockout mice as compared with injection of wild-type littermates. Moreover, local injection of an adenoviral vector encoding LIF suppresses both measures of inflammation. In contrast, injection of an adenoviral vector encoding β-galactosidase has no discernable effect on inflammation. In addition, comparison of the CFA responses in LIF knockout vs wild-type skin reveals that LIF is an important regulator of IL-1β, IL-6, IL-7, IL-2Rα, and IFN-γ in cutaneous inflammation. These and our previous data indicate that both endogenous and exogenous LIF are anti-inflammatory in the CFA model and that LIF is a key regulator of the cytokine cascade. The results also indicate that adenoviral gene delivery can be an effective therapeutic approach in this paradigm.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/ 10.4049/​jimmunol.166.3.2049DOIArticle
http://www.jimmunol.org/content/166/3/2049PublisherArticle
Additional Information:© 2001 by The American Association of Immunologists. Received July 20, 2000. Accepted November 8, 2000. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. This work was supported by a grant from the National Institute of Neurological Disorders and Stroke (to P.H.P.) and a fellowship from the Korean Ministry of Science and Technology (to S.C.L.). We thank D. McDowell for laboratory administration, J. Baer for help and advice with animal care, and R. Sowmayalakshmi and S. Schwarz for comments on this manuscript
Funders:
Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
Ministry of Science and Technology (Korea)UNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20160210-145408606
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160210-145408606
Official Citation:Studies Using Leukemia Inhibitory Factor (LIF) Knockout Mice and a LIF Adenoviral Vector Demonstrate a Key Anti-Inflammatory Role for This Cytokine in Cutaneous Inflammation Min Zhu, Kazuhiko Oishi, Sang Chul Lee, and Paul H. Patterson J Immunol 2001 166:2049-2054; doi:10.4049/jimmunol.166.3.2049
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64386
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Feb 2016 23:47
Last Modified:03 Oct 2019 09:37

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