A Caltech Library Service

In Vivo Trans-Synaptic Tract Tracing From the Murine Striatum and Amygdala Utilizing Manganese Enhanced MRI (MEMRI)

Pautler, Robia G. and Mongeau, Raymond and Jacobs, Russell E. (2003) In Vivo Trans-Synaptic Tract Tracing From the Murine Striatum and Amygdala Utilizing Manganese Enhanced MRI (MEMRI). Magnetic Resonance in Medicine, 50 (1). pp. 33-39. ISSN 1084-7529. doi:10.1002/mrm.10498.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


Small focal injections of manganese ion (Mn^(2+)) deep within the mouse central nervous system combined with in vivo high-resolution MRI delineate neuronal tracts originating from the site of injection. Previous work has shown that Mn^(2+) can be taken up through voltage-gated Ca^(2+) channels, transported along axons, and across synapses. Moreover, Mn^(2+) is a paramagnetic MRI contrast agent, causing positive contrast enhancement in tissues where it has accumulated. These combined properties allow for its use as an effective MRI detectable neuronal tract tracer. Injections of low concentrations of MnCl_2 into either the striatum or amygdala produced significant contrast enhancement along the known neuronal circuitry. The observed enhancement pattern is different at each injection site and enhancement of the homotopic areas was observed in both cases. Ten days postinjection, the Mn^(2+) had washed out, as evidenced by the absence of positive contrast enhancement within the brain. This methodology allows imaging of neuronal tracts long after the injection of the ion because Mn^(2+) concentrates in active neurons and resides for extended periods of time. With appropriate controls, differentiation of subsets of neuronal pathways associated with behavioral and pharmacological paradigms should be feasible.

Item Type:Article
Related URLs:
URLURL TypeDescription
Jacobs, Russell E.0000-0002-1382-8486
Additional Information:© 2003 Wiley-Liss, Inc. Received 10 July 2002; revised 13 February 2003; accepted 13 February 2003; article first published online: 13 Jun 2003. Grant sponsors: Beckman Institute; National Institute of Child Health and Human Development; Grant number: HD25390; Grant sponsor: National Center for Research Resources; Grant number: RR13625; Grant sponsor: National Institute of Mental Health; Grant number: MH61223; Grant sponsor: Human Brain Project funded jointly by the National Institute of Bioimaging and Bioengineering and the National Institute of Mental Health; Grant number: EB00232. We thank Sigrid Shwartz for invaluable help with the stereotaxic injections and Helen McBride, Angelique Louie, and J. Michael Tyszka for comments on the preparation of the manuscript.
Funding AgencyGrant Number
Caltech Beckman InstituteUNSPECIFIED
National Center for Research Resources (NCRR)RR13625
National Institute of Mental Health (NIMH)MH61223
National Institute of Biomedical Imaging and BioengineeringEB00232
Subject Keywords:Mn^(2+); mouse brain; tract tracing; MRI; trans-synaptic; amygdala; striatum
Issue or Number:1
Record Number:CaltechAUTHORS:20160212-083653243
Persistent URL:
Official Citation:Pautler, R. G., Mongeau, R. and Jacobs, R. E. (2003), In vivo trans-synaptic tract tracing from the murine striatum and amygdala utilizing manganese enhanced MRI (MEMRI). Magn Reson Med, 50: 33–39. doi: 10.1002/mrm.10498
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64452
Deposited By: Melissa Ray
Deposited On:19 Feb 2016 20:50
Last Modified:10 Nov 2021 23:31

Repository Staff Only: item control page