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Migratory Patterns and Developmental Potential of Trunk Neural Crest Cells in the Axolotl Embryo

Epperlein, Hans-Henning and Selleck, Mark A. J. and Meulemans, Daniel and Mchedlishvili, Levan and Cerny, Robert and Sobkow, Lidia and Bronner-Fraser, Marianne (2007) Migratory Patterns and Developmental Potential of Trunk Neural Crest Cells in the Axolotl Embryo. Developmental Dynamics, 236 (2). pp. 389-403. ISSN 1058-8388. https://resolver.caltech.edu/CaltechAUTHORS:20160212-100711722

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Abstract

Using cell markers and grafting, we examined the timing of migration and developmental potential of trunk neural crest cells in axolotl. No obvious differences in pathway choice were noted for DiI-labeling at different lateral or medial positions of the trunk neural folds in neurulae, which contributed not only to neural crest but also to Rohon-Beard neurons. Labeling wild-type dorsal trunks at pre- and early-migratory stages revealed that individual neural crest cells migrate away from the neural tube along two main routes: first, dorsolaterally between the epidermis and somites and, later, ventromedially between the somites and neural tube/notochord. Dorsolaterally migrating crest primarily forms pigment cells, with those from anterior (but not mid or posterior) trunk neural folds also contributing glia and neurons to the lateral line. White mutants have impaired dorsolateral but normal ventromedial migration. At late migratory stages, most labeled cells move along the ventromedial pathway or into the dorsal fin. Contrasting with other anamniotes, axolotl has a minor neural crest contribution to the dorsal fin, most of which arises from the dermomyotome. Taken together, the results reveal stereotypic migration and differentiation of neural crest cells in axolotl that differ from other vertebrates in timing of entry onto the dorsolateral pathway and extent of contribution to some derivatives.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/dvdy.21039DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.21039/abstractPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2006 Wiley-Liss, Inc. Accepted 31 October 2006. Published online 19 December 2006. Article first published online: 20 Dec 2006. This research was supported by DFG (EP8/7-1) to H.H.E., by NS051051 to M.B.F., and a James H. Zumberge Research and Innovation Fund and a Donald E. and Delia B. Baxter Foundation award to M.A.J.S. H.H.E. thanks G. Northcutt (San Diego) and G. Schlosser (Bremen) for valuable information on the lateral line, I. Beck, P. Mirtschink, F. Heidrich, and T. Schwalm for analysis of cell migration or computer work, and S. Bramke for histology.
Funders:
Funding AgencyGrant Number
Deutsche Forschungsgemeinschaft (DFG)Ep8/7-1
NIHNS051051
James H. Zumberge Research and Innovation FundUNSPECIFIED
Donald E. and Delia B. Baxter FoundationUNSPECIFIED
Subject Keywords:DiI; fluorescent dextrans; GFP transgenic embryos; trunk neural crest; migration; dorsal fin; Rohon-Beard cells; lateral line; axolotl
Issue or Number:2
Record Number:CaltechAUTHORS:20160212-100711722
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160212-100711722
Official Citation:Epperlein, H.-H., Selleck, M. A.J., Meulemans, D., Mchedlishvili, L., Cerny, R., Sobkow, L. and Bronner-Fraser, M. (2007), Migratory patterns and developmental potential of trunk neural crest cells in the axolotl embryo. Dev. Dyn., 236: 389–403. doi: 10.1002/dvdy.21039
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64466
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:19 Feb 2016 20:32
Last Modified:03 Oct 2019 09:38

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