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Two-Dimensional and Three-Dimensional Time-Lapse Microscopic Magnetic Resonance Imaging of Xenopus Gastrulation Movements Using Intrinsic Tissue-Specific Contrast

Papan, Cyrus and Boulat, Benoit and Velan, S. Sendhil and Fraser, Scott E. and Jacobs, Russell E. (2007) Two-Dimensional and Three-Dimensional Time-Lapse Microscopic Magnetic Resonance Imaging of Xenopus Gastrulation Movements Using Intrinsic Tissue-Specific Contrast. Developmental Dynamics, 236 (2). pp. 494-501. ISSN 1058-8388. https://resolver.caltech.edu/CaltechAUTHORS:20160212-102203764

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Abstract

The amphibian embryo undergoes radical tissue transformations during blastula and gastrula stages, but live observation of internal morphogenetic events by optical microscopy is not feasible due to the opacity of the early embryo. Here, we report on the use of microscopic magnetic resonance imaging (MRI) to directly follow morphogenetic movements during blastula and gastrula stages of the Xenopus laevis embryo. We compare three different MRI modalities that take advantage of the intrinsic contrast present in embryonic tissues: three-dimensional (3D) fat-imaging, 3D water-imaging, and 2D high-speed high-resolution imaging of early embryonic stages. We show that the features revealed by the intrinsic contrast correlate with the histological structure of the embryo. Using this tissue specific intrinsic contrast, the main embryonic tissues and internal tissue movements as well as archenteron invagination can be differentiated without cell labeling. We present 2D and 3D time-lapse sequences of early Xenopus embryonic development, spanning the stages from early blastula to the end of gastrula, which show the complex internal rearrangements of gastrulation in essentially real-time.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/dvdy.21045DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.21045/abstractPublisherArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.21045/suppinfoRelated ItemSupplementary Information
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Jacobs, Russell E.0000-0002-1382-8486
Additional Information:© 2006 Wiley-Liss, Inc. Accepted 14 November 2006. Article first published online: 26 Dec 2006. We thank Andrey Demyanenko for coil development and Mike J. Tyszka, Seth Ruffins, and Rachel S. Kraut for critical discussions and suggestions. Funded by NCRR-BIRN. Grant Number: NIH RR13642 Deutsche Forschungsgemeinschaft. Grant Number: PA 562/1-1.
Funders:
Funding AgencyGrant Number
National Center for Research Resources (NCRR)RR13642
Deutsche Forschungsgemeinschaft (DFG)PA 562/1-1
Subject Keywords:Xenopus laevis; gastrula; MRI; longitudinal study; 3D imaging; morphogenesis
Issue or Number:2
Record Number:CaltechAUTHORS:20160212-102203764
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160212-102203764
Official Citation:Papan, C., Boulat, B., Velan, S. S., Fraser, S. E. and Jacobs, R. E. (2007), Two-dimensional and three-dimensional time-lapse microscopic magnetic resonance imaging of Xenopus gastrulation movements using intrinsic tissue-specific contrast. Dev. Dyn., 236: 494–501. doi: 10.1002/dvdy.21045
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64468
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:19 Feb 2016 20:28
Last Modified:03 Oct 2019 09:38

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