CaltechAUTHORS
  A Caltech Library Service

Discovery of transcription factors and other candidate regulators of neural crest development

Adams, Meghan S. and Gammill, Laura S. and Bronner-Fraser, Marianne (2008) Discovery of transcription factors and other candidate regulators of neural crest development. Developmental Dynamics, 237 (4). pp. 1021-1033. ISSN 1058-8388. doi:10.1002/dvdy.21513. https://resolver.caltech.edu/CaltechAUTHORS:20160212-120927931

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160212-120927931

Abstract

Neural crest cells migrate long distances and form divergent derivatives in vertebrate embryos. Despite previous efforts to identify genes up-regulated in neural crest populations, transcription factors have proved to be elusive due to relatively low expression levels and often transient expression. We screened newly induced neural crest cells for early target genes with the aim of identifying transcriptional regulators and other developmentally important genes. This yielded numerous candidate regulators, including 14 transcription factors, many of which were not previously associated with neural crest development. Quantitative real-time polymerase chain reaction confirmed up-regulation of several transcription factors in newly induced neural crest populations in vitro. In a secondary screen by in situ hybridization, we verified the expression of >100 genes in the neural crest. We note that several of the transcription factors and other genes from the screen are expressed in other migratory cell populations and have been implicated in diverse forms of cancer.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/dvdy.21513DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.21513/abstractPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2008 Wiley-Liss, Inc. Accepted 11 February 2008; article first published online: 19 Mar 2008. We would like to thank Tatjana Sauka-Spengler and Lisa Taneyhill for technical guidance and the Bronner-Fraser lab for helpful discussions. This work was supported by grants from the NIH (DE015309 to L. S. G. and NS36585 to M. B. F.) as well as the Betty and Gordon Moore Fellowship (M. S. A.).
Funders:
Funding AgencyGrant Number
NIHDE015309
NIHNS36585
Gordon and Betty Moore FoundationUNSPECIFIED
Subject Keywords:neural crest; transcription factors; chick; specification; migration; QPCR; EMT
Issue or Number:4
DOI:10.1002/dvdy.21513
Record Number:CaltechAUTHORS:20160212-120927931
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160212-120927931
Official Citation:Adams, M. S., Gammill, L. S. and Bronner-Fraser, M. (2008), Discovery of transcription factors and other candidate regulators of neural crest development. Dev. Dyn., 237: 1021–1033. doi: 10.1002/dvdy.21513
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64476
Collection:CaltechAUTHORS
Deposited By: Melissa Ray
Deposited On:19 Feb 2016 20:30
Last Modified:10 Nov 2021 23:31

Repository Staff Only: item control page