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Discovery of transcription factors and other candidate regulators of neural crest development

Adams, Meghan S. and Gammill, Laura S. and Bronner-Fraser, Marianne (2008) Discovery of transcription factors and other candidate regulators of neural crest development. Developmental Dynamics, 237 (4). pp. 1021-1033. ISSN 1058-8388. doi:10.1002/dvdy.21513.

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Neural crest cells migrate long distances and form divergent derivatives in vertebrate embryos. Despite previous efforts to identify genes up-regulated in neural crest populations, transcription factors have proved to be elusive due to relatively low expression levels and often transient expression. We screened newly induced neural crest cells for early target genes with the aim of identifying transcriptional regulators and other developmentally important genes. This yielded numerous candidate regulators, including 14 transcription factors, many of which were not previously associated with neural crest development. Quantitative real-time polymerase chain reaction confirmed up-regulation of several transcription factors in newly induced neural crest populations in vitro. In a secondary screen by in situ hybridization, we verified the expression of >100 genes in the neural crest. We note that several of the transcription factors and other genes from the screen are expressed in other migratory cell populations and have been implicated in diverse forms of cancer.

Item Type:Article
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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2008 Wiley-Liss, Inc. Accepted 11 February 2008; article first published online: 19 Mar 2008. We would like to thank Tatjana Sauka-Spengler and Lisa Taneyhill for technical guidance and the Bronner-Fraser lab for helpful discussions. This work was supported by grants from the NIH (DE015309 to L. S. G. and NS36585 to M. B. F.) as well as the Betty and Gordon Moore Fellowship (M. S. A.).
Funding AgencyGrant Number
Gordon and Betty Moore FoundationUNSPECIFIED
Subject Keywords:neural crest; transcription factors; chick; specification; migration; QPCR; EMT
Issue or Number:4
Record Number:CaltechAUTHORS:20160212-120927931
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Official Citation:Adams, M. S., Gammill, L. S. and Bronner-Fraser, M. (2008), Discovery of transcription factors and other candidate regulators of neural crest development. Dev. Dyn., 237: 1021–1033. doi: 10.1002/dvdy.21513
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64476
Deposited By: Melissa Ray
Deposited On:19 Feb 2016 20:30
Last Modified:10 Nov 2021 23:31

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