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Precocious Terminal Differentiation of Premigratory Limb Muscle Precursor Cells Requires Positive Signalling

Venters, Sara J. and Argent, Rebecca E. and Deegan, Fiona M. and Perez-Baron, Gina and Wong, Ted S. and Tidyman, William E. and Denetclaw, Wilfred F. and Marcelle, Christophe and Bronner-Fraser, Marianne and Ordahl, Charles P. (2004) Precocious Terminal Differentiation of Premigratory Limb Muscle Precursor Cells Requires Positive Signalling. Developmental Dynamics, 229 (3). pp. 591-599. ISSN 1058-8388. doi:10.1002/dvdy.20016.

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The timing of myogenic differentiation of hypaxial muscle precursor cells in the somite lags behind that of epaxial precursors. Two hypotheses have been proposed to explain this delay. One attributes the delay to the presence of negative-acting signals from the lateral plate mesoderm adjacent to the hypaxial muscle precursor cells located in the ventrolateral lip of the somitic dermomyotome (Pourquié et al. [1995] Proc. Natl. Acad. Sci. USA 92:3219–3223). The second attributes the delay to an absence of positive-acting inductive signals, similar to those from the axial structures that induce epaxial myotome development (Pownall et al. [1996] Development 122:1475–1488). Because both studies relied principally upon changes in the expression pattern of mRNAs specific to early muscle precursor cell markers, we revisited these experiments using two methods to assess muscle terminal differentiation. First, injection of fluorescent dyes before surgery was used to determine whether ventrolateral lip cells transform from epithelial cells to elongated myocytes. Second, an antibody to a terminal differentiation marker and a new monoclonal antibody that recognises avian and mammalian Pax3 were used for immunohistochemistry to assess the transition from precursor cell to myocyte. The results support both hypotheses and show further that placing axial structures adjacent to the somite ventrolateral lip induces an axial pattern of myocyte terminal differentiation and elongation.

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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2004 Wiley-Liss, Inc. Received 1 September 2003; Revised 14 November 2003; Accepted 14 November 2003. The authors thank Kim-Uyen Le and Nina Konstanian for technical support. C.P.O. and M.B.F. received funding from the NIH and C.P.O. and S.J.V. received funding from the Muscular Dystrophy Association. Funded by: NIH. Grant Numbers: RO1.AR-44483, NS41070. Muscular Dystrophy Association of America.
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Muscular Dystrophy Association of AmericaUNSPECIFIED
Subject Keywords:somite; hypaxial; limb; muscle; differentiation; signalling; embryo
Issue or Number:3
Record Number:CaltechAUTHORS:20160212-131244590
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Official Citation:Venters, S. J., Argent, R. E., Deegan, F. M., Perez-Baron, G., Wong, T. S., Tidyman, W. E., Denetclaw, W. F., Marcelle, C., Bronner-Fraser, M. and Ordahl, C. P. (2004), Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling. Dev. Dyn., 229: 591–599. doi: 10.1002/dvdy.20016
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64478
Deposited By: Ruth Sustaita
Deposited On:19 Feb 2016 20:11
Last Modified:10 Nov 2021 23:31

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