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Two-Point Stretchable Electrode Array for Endoluminal Electrochemical Impedance Spectroscopy Measurements of Lipid-Laden Atherosclerotic Plaques

Sevag Packard, René R. and Zhang, Xiaoxiao and Luo, Yuan and Ma, Teng and Jen, Nelson and Ma, Jianguo and Demer, Linda L. and Zhou, Qifa and Sayre, James W. and Li, Rongsong and Tai, Yu-Chong and Hsiai, Tzung K. (2016) Two-Point Stretchable Electrode Array for Endoluminal Electrochemical Impedance Spectroscopy Measurements of Lipid-Laden Atherosclerotic Plaques. Annals of Biomedical Engineering, 44 (9). pp. 2695-2706. ISSN 0090-6964. http://resolver.caltech.edu/CaltechAUTHORS:20160217-102637017

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Abstract

Four-point electrode systems are commonly used for electric impedance measurements of biomaterials and tissues. We introduce a 2-point system to reduce electrode polarization for heterogeneous measurements of vascular wall. Presence of endoluminal oxidized low density lipoprotein (oxLDL) and lipids alters the electrochemical impedance that can be measured by electrochemical impedance spectroscopy (EIS). We developed a catheter-based 2-point micro-electrode configuration for intravascular deployment in New Zealand White rabbits. An array of 2 flexible round electrodes, 240 µm in diameter and separated by 400 µm was microfabricated and mounted on an inflatable balloon catheter for EIS measurement of the oxLDL-rich lesions developed as a result of high-fat diet-induced hyperlipidemia. Upon balloon inflation, the 2-point electrode array conformed to the arterial wall to allow deep intraplaque penetration via alternating current (AC). The frequency sweep from 10 to 300 kHz generated an increase in capacitance, providing distinct changes in both impedance (Ω) and phase (ϕ) in relation to varying degrees of intraplaque lipid burden in the aorta. Aortic endoluminal EIS measurements were compared with epicardial fat tissue and validated by intravascular ultrasound and immunohistochemistry for plaque lipids and foam cells. Thus, we demonstrate a new approach to quantify endoluminal EIS via a 2-point stretchable electrode strategy.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s10439-016-1559-9DOIArticle
http://link.springer.com/article/10.1007%2Fs10439-016-1559-9PublisherArticle
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2016 Biomedical Engineering Society. Received 19 November 2015; accepted 29 January 2016. First online: 08 February 2016. Associate Editor Scott I. Simon oversaw the review of this article. The present work was funded by National Institutes of Health grants HL118650 (T.K.H.), HL083015 (T.K.H.), HD069305 (T.K.H.), HL111437 (T.K.H.), T32HL007895 (R.R.S.P.) and the UCLA STAR program (R.R.S.P.). The authors have no conflict of interest to disclose.
Funders:
Funding AgencyGrant Number
NIHHL118650
NIHHL083015
NIHHD069305
NIHHL111437
NIHT32HL007895
UCLA STAR programUNSPECIFIED
Subject Keywords:Electrochemical impedance spectroscopy, Flexible electronics, Lipid-rich plaque, Atherosclerosis
Record Number:CaltechAUTHORS:20160217-102637017
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160217-102637017
Official Citation:Packard, R.R.S., Zhang, X., Luo, Y. et al. Ann Biomed Eng (2016) 44: 2695. doi:10.1007/s10439-016-1559-9
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64532
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Feb 2016 21:40
Last Modified:18 Aug 2016 21:21

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