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DNA Electrochemistry as a Probe of Base Pair Stacking in A-, B-, and Z-Form DNA

Boon, Elizabeth M. and Barton, Jacqueline K. (2003) DNA Electrochemistry as a Probe of Base Pair Stacking in A-, B-, and Z-Form DNA. Bioconjugate Chemistry, 14 (6). pp. 1140-1147. ISSN 1043-1802. doi:10.1021/bc034139l.

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DNA-mediated charge transport (CT) chemistry is sensitive to DNA structure and base pair stacking. In an electrochemical assay based upon DNA CT, DNA-modified electrode surfaces are used to examine the electrochemical reduction of methylene blue (MB), a small molecule that binds to the DNA film by intercalation. Here electrochemically we probe CT in the three primary conformations of double-stranded nucleic acids, A-, B-, and Z-form DNA. The A-form is examined in the context of a DNA/RNA hybrid duplex and Z-DNA, in duplexes containing d(^(m)CG)_8 sequences at high Mg^(2+) concentrations. We find that both A- and B-DNA support efficient DNA CT as measured by MB reduction in the DNA film; a lower level of reduction is evident with the Z-form film. Furthermore, mismatches incorporated into A-form duplexes, as in B-form duplexes, disrupt MB reduction, thus providing a strategy for mutation detection through testing of RNA transcripts at DNA electrodes.

Item Type:Article
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URLURL TypeDescription
Boon, Elizabeth M.0000-0003-1891-839X
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2003 American Chemical Society. Received August 5, 2003; Revised Manuscript Received September 11, 2003; Publication Date (Web): October 24, 2003. We are grateful to the National Institutes of Health (GM61077) for their financial support of this research and to Melanie O’Neill for assistance with DNA figures.
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Issue or Number:6
Record Number:CaltechAUTHORS:20160219-115028115
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Official Citation:DNA Electrochemistry as a Probe of Base Pair Stacking in A-, B-, and Z-Form DNA Elizabeth M. Boon and Jacqueline K. Barton Bioconjugate Chemistry 2003 14 (6), 1140-1147 DOI: 10.1021/bc034139l
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64609
Deposited By: Melissa Ray
Deposited On:19 Feb 2016 20:25
Last Modified:10 Nov 2021 23:33

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