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Linking chlorophyll a fluorescence to photosynthesis for remote sensing applications: mechanisms and challenges

Porcar-Castell, Albert and Tyystjärvi, Esa and Atherton, Jon and van der Tol, Christiaan and Flexas, Jaume and Pfündel, Erhard E. and Moreno, Jose and Frankenberg, Christian and Berry, Joseph A. (2014) Linking chlorophyll a fluorescence to photosynthesis for remote sensing applications: mechanisms and challenges. Journal of Experimental Botany, 65 (15). pp. 4065-4095. ISSN 0022-0957. doi:10.1093/jxb/eru191.

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subcellular levels. ChlF is now measurable from remote sensing platforms. This provides a new optical means to track photosynthesis and gross primary productivity of terrestrial ecosystems. Importantly, the spatiotemporal and methodological context of the new applications is dramatically different compared with most of the available ChlF literature, which raises a number of important considerations. Although we have a good mechanistic understanding of the processes that control the ChlF signal over the short term, the seasonal link between ChlF and photosynthesis remains obscure. Additionally, while the current understanding of in vivo ChlF is based on pulse amplitude-modulated (PAM) measurements, remote sensing applications are based on the measurement of the passive solar-induced chlorophyll fluorescence (SIF), which entails important differences and new challenges that remain to be solved. In this review we introduce and revisit the physical, physiological, and methodological factors that control the leaf-level ChlF signal in the context of the new remote sensing applications. Specifically, we present the basis of photosynthetic acclimation and its optical signals, we introduce the physical and physiological basis of ChlF from the molecular to the leaf level and beyond, and we introduce and compare PAM and SIF methodology. Finally, we evaluate and identify the challenges that still remain to be answered in order to consolidate our mechanistic understanding of the remotely sensed SIF signal.

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Frankenberg, Christian0000-0002-0546-5857
Additional Information:© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. Received 14 February 2014; Revised 21 March 2014; Accepted 31 March 2014. First published online: May 27, 2014. This work has been supported by the Academy of Finland [grant nos 1138884 and 272041 to AP-C and 259075 to ET], and the University of Helsinki (grant no 490116). This review idea originated from discussions during a Workshop organized by the Keck Institute for Space Studies ‘New Methods for Measurements of Photosynthesis from Space’. The availability of results from ongoing preparatory studies for the ESA FLEX mission is acknowledged. We thank Professor Kari Heliovaara for help in preparing Figs 2, 4, and 7.
Group:Keck Institute for Space Studies
Funding AgencyGrant Number
Academy of Finland1138884
Academy of Finland272041
Academy of Finland259075
University of Helsinki490116
Keck Institute for Space Studies (KISS)UNSPECIFIED
Subject Keywords:Gross primary production, GPP, leaf level, photosystem II, photosystem I, PSII, PSI, photosynthesis dynamics, pulse amplitude modulation, PAM, PSII connectivity, remote sensing, solar-induced fluorescence, sun-induced fluorescence, SIF
Issue or Number:15
Record Number:CaltechAUTHORS:20160219-150631984
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Official Citation:Albert Porcar-Castell, Esa Tyystjärvi, Jon Atherton, Christiaan van der Tol, Jaume Flexas, Erhard E. Pfündel, Jose Moreno, Christian Frankenberg, and Joseph A. Berry Linking chlorophyll a fluorescence to photosynthesis for remote sensing applications: mechanisms and challenges J. Exp. Bot. (2014) 65 (15): 4065-4095 first published online May 27, 2014 doi:10.1093/jxb/eru191
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64613
Deposited By: Colette Connor
Deposited On:22 Feb 2016 18:34
Last Modified:10 Nov 2021 23:33

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