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Antiidiotypic antibodies as probes for the Sindbis virus receptor

Wang, Kang-Sheng and Schmaljohn, Alan L. and Kuhn, Richard J. and Strauss, James H. (1991) Antiidiotypic antibodies as probes for the Sindbis virus receptor. Virology, 181 (2). pp. 694-702. ISSN 0042-6822. https://resolver.caltech.edu/CaltechAUTHORS:20160222-154455916

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Abstract

Rabbit polyclonal antiidiotypic antibodies were made to mouse monoclonal antibodies that neutralize the infectivity of Sindbis virus. One of the antiidiotypic antisera obtained has properties characteristic of an anti receptor antiserum. It binds to the surface of chicken cells as shown by immunofluorescence and partially blocks virus binding to these cells as determined by binding of radio-labeled virus or by a plaque reduction assay. It also immunoprecipitates a protein with a molecular weight of 63,000 from chicken cells. From the fact that the antiserum will only partially block virus uptake, and that it does not block uptake of a variant of Sindbis virus resistant to the monoclonal antibody used to produce the antiidiotypic antiserum, we propose that at least two distinguishable receptors can be used by Sindbis virus to enter chicken cells. Furthermore, the receptors used by Sindbis to enter BHK cells appear to be different from those on chicken cells, at least in part, in that the antiidiotypic antiserum does not recognize the BHK counterpart of the chicken cell receptor. We suggest that the alphaviruses use a number of distinguishable receptors which differ depending on the host and the tissue. In chicken cells the 63,000 molecular weight protein may be one of them. The diversity of such multiple receptors could account for the very wide host range of the alphaviruses, which infect mosquitoes, birds, and mammals.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0042-6822(91)90903-ODOIArticle
http://www.sciencedirect.com/science/article/pii/004268229190903OPublisherArticle
Additional Information:© 1991 Academic Press, Inc. Received November 16, 1990; accepted January 7, 1991. We are grateful to E. Rothenberg and E. Strauss for many stimulating discussions and for help in preparing the manuscript, and to R. Diamond for help with the FACS analysis. This work was supported by NIH Grant AI 20612 to J.H.S. Early portions of the a-Id work were supported by Office of Naval Research Contract N00014-84-K-0536 to A.L.S.
Funders:
Funding AgencyGrant Number
NIHAI 20612
Office of Naval Research (ONR)N00014-84-K-0536
Issue or Number:2
Record Number:CaltechAUTHORS:20160222-154455916
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160222-154455916
Official Citation:Kang-Sheng Wang, Alan L Schmaljohn, Richard J. Kuhn, James H. Strauss, Antiidiotypic antibodies as probes for the sindbis virus receptor, Virology, Volume 181, Issue 2, 1991, Pages 694-702, ISSN 0042-6822, http://dx.doi.org/10.1016/0042-6822(91)90903-O. (http://www.sciencedirect.com/science/article/pii/004268229190903O)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64662
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:23 Feb 2016 00:51
Last Modified:03 Oct 2019 09:40

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