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Wide field-of-view fluorescence image deconvolution with aberration-estimation from Fourier ptychography

Chung, Jaebum and Kim, Jinho and Ou, Xiaoze and Horstmeyer, Roarke and Yang, Changhuei (2016) Wide field-of-view fluorescence image deconvolution with aberration-estimation from Fourier ptychography. Biomedical Optics Express, 7 (2). pp. 352-368. ISSN 2156-7085. PMCID PMC4771454.

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This paper presents a method to simultaneously acquire an aberration-corrected, wide field-of-view fluorescence image and a high-resolution coherent bright-field image using a computational microscopy method. First, the procedure applies Fourier ptychographic microscopy (FPM) to retrieve the amplitude and phase of a sample, at a resolution that significantly exceeds the cutoff spatial frequency of the microscope objective lens. At the same time, redundancy within the set of acquired FPM bright-field images offers a means to estimate microscope aberrations. Second, the procedure acquires an aberrated fluorescence image, and computationally improves its resolution through deconvolution with the estimated aberration map. An experimental demonstration successfully improves the bright-field resolution of fixed, stained and fluorescently tagged HeLa cells by a factor of 4.9, and reduces the error caused by aberrations in a fluorescence image by up to 31%, over a field of view of 6.2 mm by 9.3 mm. For optimal deconvolution, we show the fluorescence image needs to have a signal-to-noise ratio of at least ~18.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Chung, Jaebum0000-0003-3932-8428
Horstmeyer, Roarke0000-0002-2480-9141
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2016 Optical Society of America. Received 2 Nov 2015; revised 16 Dec 2015; accepted 29 Dec 2015; published 7 Jan 2016. This project was funded by the National Institute of Health (NIH) Agency Award: R01 AI096226; and the Caltech Innovation Initiative (CII): 25570015. We thank Mooseok Jang for helpful discussions.
Funding AgencyGrant Number
NIHR01 AI096226
Caltech Innovation Initiative (CI2)25570015
Issue or Number:2
Classification Code:OCIS codes: (180.2520) Fluorescence microscopy; (070.0070) Fourier optics and signal processing
PubMed Central ID:PMC4771454
Record Number:CaltechAUTHORS:20160225-134254700
Persistent URL:
Official Citation:J. Chung, J. Kim, X. Ou, R. Horstmeyer, and C. Yang, "Wide field-of-view fluorescence image deconvolution with aberration-estimation from Fourier ptychography," Biomed. Opt. Express 7, 352-368 (2016)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64767
Deposited By: Tony Diaz
Deposited On:25 Feb 2016 22:35
Last Modified:09 Mar 2020 13:19

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