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Inorganic−Organic Hybrid Luminescent Binary Probe for DNA Detection Based on Spin-Forbidden Resonance Energy Transfer

Martí, Angel A. and Puckett, Cindy A. and Dyer, Joanne and Stevens, Nathan and Jockusch, Steffen and Ju, Jingyue and Barton, Jacqueline K. and Turro, Nicholas J. (2007) Inorganic−Organic Hybrid Luminescent Binary Probe for DNA Detection Based on Spin-Forbidden Resonance Energy Transfer. Journal of the American Chemical Society, 129 (28). pp. 8680-8681. ISSN 0002-7863. PMCID PMC2747585. doi:10.1021/ja0717257. https://resolver.caltech.edu/CaltechAUTHORS:20160226-135926560

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Abstract

We describe the design of new fluorescent binary probe sensors for DNA detection based on spin-forbidden resonance energy transfer (SF-RET). Binary probes consist of a donor and acceptor fluorophores that are attached to two different oligonucleotides and serve as a resonance energy transfer (RET) donor−acceptor pair when hybridized to adjacent sites of a target sequence. In the absence of target, excitation of the donor results in fluorescence only from the donor, but when the probes hybridize to the target, the fluorophores are brought into close proximity favoring RET, yielding fluorescence mainly from the acceptor fluorophore. These new binary probes use the metal complex Ru(bpy‘)(DIP)_2^(2+) as the energy donor and an organic fluorophore (Cy5) as the energy acceptor. Energy transfer from the MLCT state of the Ru complex to singlet Cy5 is spin forbidden and produces a delayed fluorescence of Cy5. This paper demonstrates that fluorescence delay of Cy5 can be used to time resolve the emission of the probe from the intense fluorescence background of a model system for cellular background; this provides the reported system to overcome intense autofluorescence, an important and general advantage over “classical” spin-allowed steady-state probes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0717257DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0717257PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0717257PublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747585/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2007 American Chemical Society. Received March 12, 2007. Publication Date (Web): June 26, 2007. This work was supported by the Center of Excellence in Genomic Science Grant P50 HG002806 from the NIH and by the NSF under grant NSF CHE-0415516. J.K.B. thanks the NIH (GM33309) for financial support.
Funders:
Funding AgencyGrant Number
NIHP50 HG002806
NSFCHE-0415516
NIHGM33309
Issue or Number:28
PubMed Central ID:PMC2747585
DOI:10.1021/ja0717257
Record Number:CaltechAUTHORS:20160226-135926560
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160226-135926560
Official Citation:Inorganic−Organic Hybrid Luminescent Binary Probe for DNA Detection Based on Spin-Forbidden Resonance Energy Transfer Angel A. Martí, Cindy A. Puckett, Joanne Dyer, Nathan Stevens, Steffen Jockusch, Jingyue Ju, Jacqueline K. Barton, and Nicholas J. Turro Journal of the American Chemical Society 2007 129 (28), 8680-8681 DOI: 10.1021/ja0717257
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64808
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Feb 2016 18:27
Last Modified:10 Nov 2021 23:35

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