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Electrical Detection of TATA Binding Protein at DNA-Modified Microelectrodes

Gorodetsky, Alon A. and Ebrahim, Ali and Barton, Jacqueline K. (2008) Electrical Detection of TATA Binding Protein at DNA-Modified Microelectrodes. Journal of the American Chemical Society, 130 (10). pp. 2924-2925. ISSN 0002-7863. PMCID PMC2747583. doi:10.1021/ja7106756. https://resolver.caltech.edu/CaltechAUTHORS:20160226-135927612

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[img] PDF (Synthesis and characterization of the Nile Blue/DNA conjugate. Details of the KCl wash showing reversible TBP binding. Voltammograms after addition of lower concentrations of TBP to DNA-modified microelectrodes. Voltammograms revealing the effect ...) - Supplemental Material
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Abstract

A simple method for the electrochemical detection of TATA-binding protein is demonstrated at DNA-modified microelectrodes. The assay is general and based on the interruption of DNA-mediated charge transport to Nile Blue, a redox-active probe covalently attached to the DNA base pair stack. Nanomolar quantities of TATA binding protein can be detected on the microelectrodes even in the presence of micromolar amounts of bovine serum albumin, EndonucleaseIII, or Bam HI methyltransferase. The scheme outlined provides a basis for the sensitive electrical detection of numerous proteins on a single DNA chip.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja7106756DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja7106756PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja7106756PublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747583/PubMed CentralArticle
ORCID:
AuthorORCID
Gorodetsky, Alon A.0000-0002-3811-552X
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2008 American Chemical Society. Received November 28, 2007. Publication Date (Web): February 14, 2008. We are grateful to the NIH (GM61077) for their financial support.
Funders:
Funding AgencyGrant Number
NIHGM61077
Issue or Number:10
PubMed Central ID:PMC2747583
DOI:10.1021/ja7106756
Record Number:CaltechAUTHORS:20160226-135927612
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160226-135927612
Official Citation:Electrical Detection of TATA Binding Protein at DNA-Modified Microelectrodes Alon A. Gorodetsky, Ali Ebrahim, and Jacqueline K. Barton Journal of the American Chemical Society 2008 130 (10), 2924-2925 DOI: 10.1021/ja7106756
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64812
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Feb 2016 18:13
Last Modified:10 Nov 2021 23:35

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