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Binding of Ru(bpy)_2(eilatin)^(2+)to Matched and Mismatched DNA

Zeglis, Brian M. and Barton, Jacqueline K. (2008) Binding of Ru(bpy)_2(eilatin)^(2+)to Matched and Mismatched DNA. Inorganic Chemistry, 47 (14). pp. 6452-6457. ISSN 0020-1669. PMCID PMC2747595. doi:10.1021/ic8006537. https://resolver.caltech.edu/CaltechAUTHORS:20160229-073032218

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Abstract

The DNA-binding properties of Ru(bpy)_2(eilatin)^(2+) have been investigated to determine if the sterically expansive eilatin ligand confers specificity for destabilized single-base mismatches in DNA. Competitive DNA photocleavage experiments employing a sequence-neutral metallointercalator, Rh(bpy)_2(phi)^(3+)(phi = 9,10-phenanthrenequinonediimine), and a mismatch-specific metalloinsertor, Rh(bpy)_2(chrysi)^(3+) (chrysi = chrysene-5,6-quinonediimine), reveal that the eilatin complex binds to a CC mismatched site with an apparent binding constant of 2.2(2) × 10^6 M^(−1). Nonetheless, the selectivity in binding mismatched DNA is not high: competitive titrations with Rh(bpy)_2(phi)^(3+) show that the complex binds also to well-matched B-form sites. Thus, Ru(bpy)_2(eilatin)^(2+), despite containing the extremely expansive eilatin ligand, displays lower selectivity for the mismatch than does Rh(bpy)_2(chrysi)^(3+), a metalloinsertor containing the smaller, though still bulky, chrysene-5,6-quinonediimine ligand. In summary, the size and shape of the eilatin ligand allow stacking with both well-matched and mismatched DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ic8006537DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ic8006537PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ic8006537PublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747595/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2008 American Chemical Society. Received April 11, 2008. Publication Date (Web): June 25, 2008. We thank the NIH (Grant GM33309) for their financial support. We also thank Drs. Y. Kashman and Y. Tor for helpful correspondence.
Funders:
Funding AgencyGrant Number
NIHGM33309
Issue or Number:14
PubMed Central ID:PMC2747595
DOI:10.1021/ic8006537
Record Number:CaltechAUTHORS:20160229-073032218
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160229-073032218
Official Citation:Binding of Ru(bpy) 2(eilatin) 2+ to Matched and Mismatched DNA Brian M. Zeglis, Jacqueline K. Barton, Inorganic Chemistry 2008 47 (14), 6452-6457 DOI: 10.1021/ic8006537
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64829
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Feb 2016 17:54
Last Modified:10 Nov 2021 23:35

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