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Binding of Ru(bpy)_2(eilatin)^(2+)to Matched and Mismatched DNA

Zeglis, Brian M. and Barton, Jacqueline K. (2008) Binding of Ru(bpy)_2(eilatin)^(2+)to Matched and Mismatched DNA. Inorganic Chemistry, 47 (14). pp. 6452-6457. ISSN 0020-1669. PMCID PMC2747595. doi:10.1021/ic8006537.

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The DNA-binding properties of Ru(bpy)_2(eilatin)^(2+) have been investigated to determine if the sterically expansive eilatin ligand confers specificity for destabilized single-base mismatches in DNA. Competitive DNA photocleavage experiments employing a sequence-neutral metallointercalator, Rh(bpy)_2(phi)^(3+)(phi = 9,10-phenanthrenequinonediimine), and a mismatch-specific metalloinsertor, Rh(bpy)_2(chrysi)^(3+) (chrysi = chrysene-5,6-quinonediimine), reveal that the eilatin complex binds to a CC mismatched site with an apparent binding constant of 2.2(2) × 10^6 M^(−1). Nonetheless, the selectivity in binding mismatched DNA is not high: competitive titrations with Rh(bpy)_2(phi)^(3+) show that the complex binds also to well-matched B-form sites. Thus, Ru(bpy)_2(eilatin)^(2+), despite containing the extremely expansive eilatin ligand, displays lower selectivity for the mismatch than does Rh(bpy)_2(chrysi)^(3+), a metalloinsertor containing the smaller, though still bulky, chrysene-5,6-quinonediimine ligand. In summary, the size and shape of the eilatin ligand allow stacking with both well-matched and mismatched DNA.

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URLURL TypeDescription Information CentralArticle
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2008 American Chemical Society. Received April 11, 2008. Publication Date (Web): June 25, 2008. We thank the NIH (Grant GM33309) for their financial support. We also thank Drs. Y. Kashman and Y. Tor for helpful correspondence.
Funding AgencyGrant Number
Issue or Number:14
PubMed Central ID:PMC2747595
Record Number:CaltechAUTHORS:20160229-073032218
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Official Citation:Binding of Ru(bpy) 2(eilatin) 2+ to Matched and Mismatched DNA Brian M. Zeglis, Jacqueline K. Barton, Inorganic Chemistry 2008 47 (14), 6452-6457 DOI: 10.1021/ic8006537
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64829
Deposited By: Ruth Sustaita
Deposited On:29 Feb 2016 17:54
Last Modified:10 Nov 2021 23:35

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