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Site-Specific DNA Photocleavage by Rhodium Intercalators Analyzed by MALDI-TOF Mass Spectrometry

Brunner, Jens and Barton, Jacqueline K. (2006) Site-Specific DNA Photocleavage by Rhodium Intercalators Analyzed by MALDI-TOF Mass Spectrometry. Journal of the American Chemical Society, 128 (21). pp. 6772-6773. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160229-085221075

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Abstract

The DNA photocleavage reaction of mismatch-selective Rh complexes has been analyzed by MALDI-TOF mass spectrometry as well as gel electrophoresis analysis of radioactively tagged oligonucleotides. Analogous results are obtained with these two techniques showing site-specific cleavage neighboring the mismatch to yield primarily 5‘- and 3‘-phosphate termini. Additional intermediates and products are observed, however, using MALDI-TOF analysis. MALDI-TOF mass spectrometry is seen to be particularly powerful in the analysis of DNA cleavage by site-specific molecules. The method requires no radioactive labeling, only little material, and analysis can be accomplished within minutes. Moreover, this mass spectral analysis of DNA cleavage yields direct information regarding products rather than simply the base pair site of cleavage.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0612753DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0612753PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0612753PublisherSupporting Information
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2006 American Chemical Society. Received February 22, 2006. Publication Date (Web): May 4, 2006. We thank the NIH (GM33309) for financial support, and the German Academic Exchange Service (DAAD) for a postdoctoral fellowship to J.B.
Funders:
Funding AgencyGrant Number
NIHGM33309
Deutscher Akademischer Austauschdienst (DAAD)UNSPECIFIED
Issue or Number:21
Record Number:CaltechAUTHORS:20160229-085221075
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160229-085221075
Official Citation:Site-Specific DNA Photocleavage by Rhodium Intercalators Analyzed by MALDI-TOF Mass Spectrometry Jens Brunner and Jacqueline K. Barton Journal of the American Chemical Society 2006 128 (21), 6772-6773 DOI: 10.1021/ja0612753
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64834
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Feb 2016 17:46
Last Modified:03 Oct 2019 09:41

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