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Electrochemistry Using Self-Assembled DNA Monolayers on Highly Oriented Pyrolytic Graphite

Gorodetsky, Alon A. and Barton, Jacqueline K. (2006) Electrochemistry Using Self-Assembled DNA Monolayers on Highly Oriented Pyrolytic Graphite. Langmuir, 22 (18). pp. 7917-7922. ISSN 0743-7463. https://resolver.caltech.edu/CaltechAUTHORS:20160229-085222529

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Abstract

Duplex DNA functionalized with pyrene has been utilized to fabricate DNA-modified electrodes on highly oriented pyrolytic graphite (HOPG). Films have been characterized using AFM and radioactive labeling as well as electrochemically. The data obtained are consistent with a close-packed structure in the film with helices oriented in a nearly upright orientation, as seen earlier with the fabrication of thiol-tethered duplexes on gold. Also as on gold, we observe the reduction of DNA-bound intercalators in a DNA-mediated reaction. The reduction of the intercalator is attenuated in the presence of the single-base mismatches, CA and GT, independent of the sequence composition of the oligonucleotide. This sensitivity to single-base mismatches is enhanced when methylene blue reduction is coupled in an electrocatalytic cycle with ferricyanide. The extended potential range afforded by the HOPG surface has allowed us also to investigate the electrochemistry of previously inaccessible metallointercalators, Ru(bpy)_2dppz^(2+) and Os(phen)_2dppz^(2+), at the DNA-modified HOPG surface. These results support the application of DNA-modified HOPG as a convenient and reproducible surface for electrochemical DNA sensors using DNA-mediated charge transport.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/la0611054DOIArticle
http://pubs.acs.org/doi/abs/10.1021/la0611054PublisherArticle
ORCID:
AuthorORCID
Gorodetsky, Alon A.0000-0002-3811-552X
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2006 American Chemical Society. Received April 24, 2006. In Final Form: June 6, 2006. Publication Date (Web): July 26, 2006. We are grateful to the NIH (Grant GM61077) for their financial support of this research. We thank Prof. C. Patrick Collier and Dr. Jinyu Chen for help with the AFM measurements.
Funders:
Funding AgencyGrant Number
NIHGM61077
Issue or Number:18
Record Number:CaltechAUTHORS:20160229-085222529
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160229-085222529
Official Citation:Electrochemistry Using Self-Assembled DNA Monolayers on Highly Oriented Pyrolytic Graphite Alon A. Gorodetsky and Jacqueline K. Barton Langmuir 2006 22 (18), 7917-7922 DOI: 10.1021/la0611054
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64839
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Feb 2016 17:48
Last Modified:03 Oct 2019 09:41

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