Gorodetsky, Alon A. and Barton, Jacqueline K. (2006) Electrochemistry Using Self-Assembled DNA Monolayers on Highly Oriented Pyrolytic Graphite. Langmuir, 22 (18). pp. 7917-7922. ISSN 0743-7463. doi:10.1021/la0611054. https://resolver.caltech.edu/CaltechAUTHORS:20160229-085222529
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160229-085222529
Abstract
Duplex DNA functionalized with pyrene has been utilized to fabricate DNA-modified electrodes on highly oriented pyrolytic graphite (HOPG). Films have been characterized using AFM and radioactive labeling as well as electrochemically. The data obtained are consistent with a close-packed structure in the film with helices oriented in a nearly upright orientation, as seen earlier with the fabrication of thiol-tethered duplexes on gold. Also as on gold, we observe the reduction of DNA-bound intercalators in a DNA-mediated reaction. The reduction of the intercalator is attenuated in the presence of the single-base mismatches, CA and GT, independent of the sequence composition of the oligonucleotide. This sensitivity to single-base mismatches is enhanced when methylene blue reduction is coupled in an electrocatalytic cycle with ferricyanide. The extended potential range afforded by the HOPG surface has allowed us also to investigate the electrochemistry of previously inaccessible metallointercalators, Ru(bpy)_2dppz^(2+) and Os(phen)_2dppz^(2+), at the DNA-modified HOPG surface. These results support the application of DNA-modified HOPG as a convenient and reproducible surface for electrochemical DNA sensors using DNA-mediated charge transport.
| Item Type: | Article | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| |||||||||
| ORCID: |
| |||||||||
| Additional Information: | © 2006 American Chemical Society. Received April 24, 2006. In Final Form: June 6, 2006. Publication Date (Web): July 26, 2006. We are grateful to the NIH (Grant GM61077) for their financial support of this research. We thank Prof. C. Patrick Collier and Dr. Jinyu Chen for help with the AFM measurements. | |||||||||
| Funders: |
| |||||||||
| Issue or Number: | 18 | |||||||||
| DOI: | 10.1021/la0611054 | |||||||||
| Record Number: | CaltechAUTHORS:20160229-085222529 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160229-085222529 | |||||||||
| Official Citation: | Electrochemistry Using Self-Assembled DNA Monolayers on Highly Oriented Pyrolytic Graphite Alon A. Gorodetsky and Jacqueline K. Barton Langmuir 2006 22 (18), 7917-7922 DOI: 10.1021/la0611054 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 64839 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 29 Feb 2016 17:48 | |||||||||
| Last Modified: | 10 Nov 2021 23:36 |
Repository Staff Only: item control page
