A Caltech Library Service

Charge Transport in DNA Duplex/Quadruplex Conjugates

Delaney, Sarah and Barton, Jacqueline K. (2003) Charge Transport in DNA Duplex/Quadruplex Conjugates. Biochemistry, 42 (48). pp. 14159-14165. ISSN 0006-2960. doi:10.1021/bi0351965.

[img] PDF (Methylation protection results for DQ-1 and DQ-2, native gel electrophoresis of duplex/quadruplex conjugates, melting temperature studies of quadruplex forming strand and duplex alone, and CT damage in the duplex/quadruplex conjugate containing an ATA...) - Supplemental Material
See Usage Policy.


Use this Persistent URL to link to this item:


DNA conjugates containing adjacent duplex and guanine quadruplex assemblies have been designed to explore charge transport into quadruplex architectures. The quadruplex assemblies have been characterized structurally using circular dichroism and by assaying for chemical protection. Using an intercalating rhodium photooxidant, noncovalently bound or tethered to the duplex end, oxidizing radicals are found to be trapped in the folded quadruplex. Damage is observed almost exclusively at the external tetrads of the quadruplex. Little damage of the center tetrad is observed, due most likely to lowered efficiency of radical trapping within the quadruplex core. This pattern of damage is distinct from that observed for repetitive G sequences within duplex DNA. The data indicate, furthermore, that in the conjugates examined, the guanine quadruplex provides a more effective trap than a 5‘-GG-3‘ guanine doublet within duplex DNA. Within these assemblies, sufficient base−base overlap must exist at the duplex/quadruplex junction to allow for charge migration. This funneling of damage to the quadruplex, as well as the unique pattern of damage within the quadruplex, requires consideration with respect to the analysis of oxidative DNA damage within the cell.

Item Type:Article
Related URLs:
URLURL TypeDescription Information
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2003 American Chemical Society. Received July 8, 2003; Revised Manuscript Received September 12, 2003. Publication Date (Web): November 13, 2003. This work was supported by NIH.
Funding AgencyGrant Number
Issue or Number:48
Record Number:CaltechAUTHORS:20160229-112429045
Persistent URL:
Official Citation:Charge Transport in DNA Duplex/Quadruplex Conjugates Sarah Delaney and Jacqueline K. Barton Biochemistry 2003 42 (48), 14159-14165 DOI: 10.1021/bi0351965
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64849
Deposited By: Ruth Sustaita
Deposited On:29 Feb 2016 19:34
Last Modified:10 Nov 2021 23:36

Repository Staff Only: item control page