CaltechAUTHORS
  A Caltech Library Service

^1H NMR Studies of Nickel(II) Complexes Bound to Oligonucleotides: A Novel Technique for Distinguishing the Binding Locations of Metal Complexes in DNA

Bhattacharya, Pratip K. and Lawson, Holly J. and Barton, Jacqueline K. (2003) ^1H NMR Studies of Nickel(II) Complexes Bound to Oligonucleotides: A Novel Technique for Distinguishing the Binding Locations of Metal Complexes in DNA. Inorganic Chemistry, 42 (26). pp. 8811-8817. ISSN 0020-1669. https://resolver.caltech.edu/CaltechAUTHORS:20160229-120833527

[img] PDF (^1H NMR spectral titrations of d(GTCGAC)_2 with [Ni(phen)_2(dppz)]Cl_2 in 90/10 H_2O/D_2O and of d(GTCGAC)_2 and d(GTGCAC)_2 with [Ni(phen)_2(phi)]Cl_2 in D_2O) - Supplemental Material
See Usage Policy.

451Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160229-120833527

Abstract

The selective paramagnetic relaxation of oligonucleotide proton resonances of d(GTCGAC)_2 and d(GTGCAC)_2 by Ni(phen)_2(L)^(2+) where L = dipyridophenazine (dppz), dipyrido[3,2-d:2‘,3‘-f]quinoxaline (dpq), and phenanthrenequinone (phi) has been examined to obtain structural insight into the noncovalent binding of these metal complexes to DNA. In the oligonucleotide d(GTCGAC)_2, preferential broadening of the G1H8, G4H8, T2H6, and C3H6 proton resonances was observed with Ni(phen)_2(dppz)^(2+), Ni(phen)_2(dpq)^(2+), and Ni(phen)_2(phi)^(2+). In the case of the sequence d(GTGCAC)_2, where the central two bases are juxtaposed from the previous one, preferential broadening was observed instead for the A5H2 proton resonance. Thus, a subtle change in the sequence of the oligonucleotide can cause significant change in the binding location of the metal complex in the oligonucleotide. Owing to comparable changes for all metal complexes and sequences in broadening of the thymine methyl proton resonances, we attribute the switch in preferential broadening to a change in site location within the oligomer rather than to an alteration of groove location. Therefore, even for DNA-binding complexes of low sequence-specificity, distinct variations in binding as a function of sequence are apparent.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ic0348291DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ic0348291PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ic0348291PublisherSupporting Information
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2003 American Chemical Society. Received July 15, 2003. Publication Date (Web): December 3, 2003. The authors gratefully acknowledge the National Institutes of Health for financial support of this research (GM33309).
Funders:
Funding AgencyGrant Number
NIHGM33309
Issue or Number:26
Record Number:CaltechAUTHORS:20160229-120833527
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160229-120833527
Official Citation:1H NMR Studies of Nickel(II) Complexes Bound to Oligonucleotides: A Novel Technique for Distinguishing the Binding Locations of Metal Complexes in DNA Pratip K. Bhattacharya, Holly J. Lawson, and Jacqueline K. Barton Inorganic Chemistry 2003 42 (26), 8811-8817 DOI: 10.1021/ic0348291
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64853
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Feb 2016 20:48
Last Modified:03 Oct 2019 09:41

Repository Staff Only: item control page