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Measuring hemodynamic changes during mammalian development

Jones, E. A. V. and Baron, M. H. and Fraser, S. E. and Dickinson, M. E. (2004) Measuring hemodynamic changes during mammalian development. American Journal of Physiology - Heart and Circulatory Physiology, 287 (4). H1561-H1569. ISSN 0363-6135. doi:10.1152/ajpheart.00081.2004. https://resolver.caltech.edu/CaltechAUTHORS:20160302-095751464

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Abstract

The pathogenesis of many congenital cardiovascular diseases involves abnormal flow within the embryonic vasculature that results either from malformations of the heart or defects in the vasculature itself. Extensive genetic and genomic analysis in mice has led to the identification of an array of mutations that result in cardiovascular defects during embryogenesis. Many of these mutations cause secondary effects within the vasculature that are thought to arise because of altered fluid dynamics. Presumably, cardiac defects disturb or reduce flow and thereby lead to the disruption of the mechanical signals necessary for proper vascular development. Unfortunately, a precise understanding of how flow disruptions lead to secondary vasculature defects has been hampered by the inadequacy of existing analytical tools. Here, we used a fast line-scanning technique for the quantitative analysis of hemodynamics during early organogenesis in mouse embryos, and we present a model system for studying cellular responses during the formation and remodeling of the mammalian cardiovascular system. Flow velocity profiles can be measured as soon as a heart begins to beat even in newly formed vessels. These studies establish a link between the pattern of blood flow within the vasculature and the stage of heart development and also enable analysis of the influence of mechanical forces during development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1152/ajpheart.00081.2004DOIArticle
http://ajpheart.physiology.org/content/287/4/H1561PublisherArticle
ORCID:
AuthorORCID
Fraser, S. E.0000-0002-5377-0223
Additional Information:© 2004 the American Physiological Society. Submitted 2 February 2004; accepted in final form 17 May 2004. The authors thank Chris Waters for technical support and Joaquin Gutierrez for assistance in animal husbandry. This work was supported by National Heart, Lung, and Blood Institute Grant R01 HL-62248 (to M. H. Baron) and Human Frontiers Science Program Grant SEF.HFSP1-1-HFSP.000002 (to S. E. Fraser). The authors also thank the Powell Foundation for partial support of this work through the Option of Bioengineering at Caltech.
Funders:
Funding AgencyGrant Number
National Heart, Lung, and Blood InstituteR01 HL-62248
Human Frontiers Science ProgramSEF.HFSP1-1-HFSP.000002
Charles Lee Powell FoundationUNSPECIFIED
Issue or Number:4
DOI:10.1152/ajpheart.00081.2004
Record Number:CaltechAUTHORS:20160302-095751464
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160302-095751464
Official Citation:Measuring hemodynamic changes during mammalian development E.A.V. Jones, M.H. Baron, S.E. Fraser, M.E. Dickinson American Journal of Physiology - Heart and Circulatory Physiology Oct 2004, 287 (4) H1561-H1569; DOI: 10.1152/ajpheart.00081.2004
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:64957
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:02 Mar 2016 18:30
Last Modified:10 Nov 2021 23:37

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