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A new view into prokaryotic cell biology from electron cryotomography

Oikonomou, Catherine M. and Jensen, Grant J. (2016) A new view into prokaryotic cell biology from electron cryotomography. Nature Reviews Microbiology, 14 (4). pp. 205-220. ISSN 1740-1526. PMCID PMC5551487. http://resolver.caltech.edu/CaltechAUTHORS:20160307-090319472

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[img] Video (QuickTime) (An intact Bdellovibrio bacteriovorus cell in standard media was plunge-frozen and imaged by ECT. The resulting tilt-series of images was reconstructed into a 3D tomogram. The movie shows the full reconstruction and segmentation, as well as fitting ...) - Supplemental Material
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Abstract

Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially native state to 'macromolecular' (~4 nm) resolution, revealing the basic architectures of complete nanomachines and their arrangements in situ. Since its inception, ECT has advanced our understanding of many aspects of prokaryotic cell biology, from morphogenesis to subcellular compartmentalization and from metabolism to complex interspecies interactions. In this Review, we highlight how ECT has provided structural and mechanistic insights into the physiology of bacteria and archaea and discuss prospects for the future.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nrmicro.2016.7DOIArticle
http://www.nature.com/nrmicro/journal/v14/n4/full/nrmicro.2016.7.htmlPublisherArticle
http://rdcu.be/gIAePublisherFree ReadCube access
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551487PubMed CentralArticle
ORCID:
AuthorORCID
Oikonomou, Catherine M.0000-0003-2312-4746
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2016 Macmillan Publishers Limited. Published online 29 February 2016. The authors apologize that they could not discuss all of the work in this burgeoning field. The authors thank members of the Jensen laboratory for helpful comments on the manuscript, and J. Ding and Y.-W. Chang for producing the accompanying movie. The authors also thank L. Sockett (University of Nottingham) for the gift of the Bdellovibrio bacteriovorus strain imaged in figure 1 and shown in the accompanying movie. Microbial electron cryotomography (ECT) in the Jensen laboatory is supported, in part, by the Howard Hughes Medical Institute, the US National Institutes of Health (grants RO1 GM101425 and RO1 GM094800), the Beckman Institute at Caltech, Caltech's Center for Environmental Microbial Interactions, and gifts to Caltech from the Gordon and Betty Moore Foundation and the Agouron Institute. The authors declare no competing interest.
Errata:It has recently come to the authors’ attention that this Review is the first place in which Figure 1 was published. Yi-Wei Chang, who collected the data for Figure 1 and helped to revise the text, should have been included as an author. Therefore, on the title page, the corrected author list should read Catherine M. Oikonomou, Yi-Wei Chang and Grant J. Jensen.
Group:Caltech Center for Environmental Microbial Interactions (CEMI)
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHRO1 GM101425
NIHRO1 GM094800
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
Caltech Center for Environmental Microbial Interactions (CEMI)UNSPECIFIED
PubMed Central ID:PMC5551487
Record Number:CaltechAUTHORS:20160307-090319472
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160307-090319472
Official Citation:A new view into prokaryotic cell biology from electron cryotomography Catherine M. Oikonomou & Grant J. Jensen Nature Reviews Microbiology 14, 205–220 (2016) doi:10.1038/nrmicro.2016.7
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65102
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Mar 2016 17:46
Last Modified:23 Apr 2019 00:41

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