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Spatial Variations in Vitreous Oxygen Consumption

Murali, Karthik and Kang, Dongyang and Nazari, Hossein and Scianmarello, Nicholas and Cadenas, Enrique and Tai, Yu-Chong and Kashani, Amir and Humayun, Mark (2016) Spatial Variations in Vitreous Oxygen Consumption. PLoS ONE, 11 (3). Art. No. e0149961. ISSN 1932-6203. PMCID PMC4773158. https://resolver.caltech.edu/CaltechAUTHORS:20160307-094844172

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Abstract

We investigated the spatial variation of vitreous oxygen consumption in enucleated porcine eyes. A custom made oxygen source was fabricated that could be localized to either the mid or posterior vitreous cavity and steady state vitreous oxygen tension was measured as a function of distance from the source using a commercially available probe. The reaction rate constant of ascorbate oxidation was estimated ex vivo by measuring the change in oxygen tension over time using vitreous harvested from porcine eyes. Vitreous ascorbate from mid and posterior vitreous was measured spectrophotometrically. When the oxygen source was placed in either the mid-vitreous (N = 6) or the posterior vitreous (N = 6), we measured a statistically significant decrease in vitreous oxygen tension as a function of distance from the oxygen source when compared to control experiments without an oxygen source; (p<0.005 for mid-vitreous and p<0.018 for posterior vitreous at all distances). The mid-vitreous oxygen tension change was significantly different from the posterior vitreous oxygen tension change at 2 and 3mm distances from the respective oxygen source (p<0.001). We also found a statistically significant lower concentration of ascorbate in the mid-vitreous as compared to posterior vitreous (p = 0.02). We determined the reaction rate constant, k = 1.61 M^(-1)s^(-1) ± 0.708 M^(-1)s^(-1) (SE), of the oxidation of ascorbate which was modeled following a second order rate equation. Our data demonstrates that vitreous oxygen consumption is higher in the posterior vitreous compared to the mid-vitreous. We also show spatial variations in vitreous ascorbate concentration.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0149961DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773158/PubMed CentralArticle
http://resolver.caltech.edu/CaltechAUTHORS:20151119-105828360Related ItemArticle
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Humayun, Mark0000-0002-5830-5208
Additional Information:© 2016 Murali et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: September 23, 2015; Accepted: February 8, 2016; Published: March 1, 2016. The authors would like to thank the National Institutes of Health, Whittier Foundation, Harrington Foundation, USC Eye Institute, Institute of Biomedical Therapeutics, and the Research to Prevent Blindness. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors have declared that no competing interests exist. Author Contributions: Conceived and designed the experiments: KM DK HN NS EC YT MH AK. Performed the experiments: KM. Analyzed the data: KM DK HN NS EC YT MH AK. Contributed reagents/materials/analysis tools: DK NS YT. Wrote the paper: KM AK.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Whittier FoundationUNSPECIFIED
Harrington FoundationUNSPECIFIED
USC Eye InstituteUNSPECIFIED
Institute of Biomedical TherapeuticsUNSPECIFIED
Research to Prevent BlindnessUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC4773158
Record Number:CaltechAUTHORS:20160307-094844172
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160307-094844172
Official Citation:Murali K, Kang D, Nazari H, Scianmarello N, Cadenas E, Tai Y-C, et al. (2016) Spatial Variations in Vitreous Oxygen Consumption. PLoS ONE 11(3): e0149961. doi:10.1371/journal.pone.0149961
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65106
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Mar 2016 17:32
Last Modified:10 Feb 2020 22:20

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