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The Cholinergic Neuronal Differentiation Factor from Heart Cell Conditioned Medium Is Different from the Cholinergic Factors in Sciatic Nerve and Spinal Cord

Rao, Mahendra S. and Landis, Story C. and Patterson, Paul H. (1990) The Cholinergic Neuronal Differentiation Factor from Heart Cell Conditioned Medium Is Different from the Cholinergic Factors in Sciatic Nerve and Spinal Cord. Developmental Biology, 139 (1). pp. 65-74. ISSN 0012-1606. doi:10.1016/0012-1606(90)90279-R.

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Environmental cues play an important role in determining the transmitter phenotype of developing sympathetic neurons. Several factors have been described which can induce cholinergic function in cultured sympathetic neurons. We have compared certain biological and immunological properties of three of them, cholinergic differentiation factor (CDF), membrane-associated neurotransmitter-stimulating factor (MANS), and ciliary neurotrophic factor (CNTF), to determine whether they are different. As previously reported, all three increased acetylcholine synthesis in cultured sympathetic neurons. In addition, MANS as well as CNTF and CDF decreased catecholamine synthesis. CNTF and MANS, but not CDF, promoted the survival of embryonic chick ciliary neurons. Affinity-purified antibodies raised against a synthetic peptide corresponding to the N-terminal sequence of CDF immunoprecipitated CDF, but not MANS or CNTF. These results indicate that although CDF, MANS, and CNTF have similar effects on transmitter synthesis by cultured sympathetic neurons, CDF lacks the ciliary neurotrophic activity of MANS and CNTF. Further, CDF possesses an N-terminal epitope which is absent from both MANS and CNTF. Thus, CDF is distinct from MANS and CNTF, and at least two factors exist which can alter the transmitter phenotype of sympathetic neurons in vitro.

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Additional Information:© 1990 Academic Press, Inc. Accepted 28 December 1989. The authors thank Drs. Sigrun Korsching and Hiro Nawa for valuable discussions and Dr. John Rudge for assistance in establishing ciliary neuron cultures. This work was supported by NINDS Javits Investigator Awards to S.C.L. and P.H.P., a Grant-in-aid from the American Heart Association to S.C.L., and a McKnight Neuroscience Research Project Award to P.H.P.
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National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
American Heart AssociationUNSPECIFIED
McKnight FoundationUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20160307-141031067
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Official Citation:Mahendra S. Rao, Story C. Landis, Paul H. Patterson, The cholinergic neuronal differentiation factor from heart cell conditioned medium is different from the cholinergic factors in sciatic nerve and spinal cord, Developmental Biology, Volume 139, Issue 1, May 1990, Pages 65-74, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65139
Deposited By: Ruth Sustaita
Deposited On:07 Mar 2016 23:27
Last Modified:10 Nov 2021 23:40

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