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Absence of Neural Crest Cells from the Region Surrounding Implanted Notochords in Situ

Pettway, Zoé and Guillory, Georgia and Bronner-Fraser, Marianne (1990) Absence of Neural Crest Cells from the Region Surrounding Implanted Notochords in Situ. Developmental Biology, 142 (2). pp. 335-345. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160307-141031592

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Abstract

Avian neural crest cells migrating along the trunk ventral pathway are distributed throughout the rostral half of the sclerotome with the exception of a neural crest cell-free space of approximately 85 μm width surrounding the notochord. To determine if this neural crest cell-free space results from the notochord inhibiting neural crest cell migration, a length of quail notochord was implanted lateral to the neural tube along the neural crest ventral migratory pathway of 2-day chicken embryos. The subsequent distribution of neural crest cells was analyzed in embryos fixed 2 days after grafting. When the donor notochord was isolated using collagenase, neural crest cells avoided the ectopic notochord and were absent from the area immediately surrounding the implant (mean distance of 43 μm). The neural crest cell-free space was significantly less when notochords were isolated using trypsin or chondroitinase digestion and was completely eliminated when notochords were fixed with paraformaldehyde or methanol prior to implantation. The implanted notochords did not appear to affect the overall number of neural crest cells, and therefore were unlikely to exert this effect by altering their viability. These results suggest that the notochord produces a substance that can inhibit neural crest cell migration and that this substance is trypsin and chondroitinase labile.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(90)90354-LDOIArticle
http://www.sciencedirect.com/science/article/pii/001216069090354LPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1990 Academic Press, Inc. Accepted 10 August 1990. We thank Dr. Scott Fraser for critical reading of the manuscript and Kristin Bruk and Mary Flowers for excellent technical assistance. This work was supported by USPHS Grant HD-15527 and a grant from the Muscular Dystrophy Association. M.B.-F. is a Sloan Foundation Fellow.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527
Muscular Dystrophy AssociationUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160307-141031592
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160307-141031592
Official Citation:Zoé Pettway, Georgia Guillory, Marianne Bronner-Fraser, Absence of neural crest cells from the region surrounding implanted notochordsin situ, Developmental Biology, Volume 142, Issue 2, December 1990, Pages 335-345, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(90)90354-L. (http://www.sciencedirect.com/science/article/pii/001216069090354L)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65141
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:08 Mar 2016 00:01
Last Modified:03 Oct 2019 09:44

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