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Differential Development of Cholinergic Neurons from Cranial and Trunk Neural Crest Cells in Vitro

Leblanc, Gabrielle G. and Epstein, Miles L. and Bronner-Fraser, Marianne E. (1990) Differential Development of Cholinergic Neurons from Cranial and Trunk Neural Crest Cells in Vitro. Developmental Biology, 137 (2). pp. 318-330. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160307-141031892

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Abstract

Several studies have suggested that the development of cholinergic properties in cranial parasympathetic neurons is determined by these cells' axial level of origin in the neural crest. All cranial parasympathetic neurons normally derive from cranial neural crest. Trunk neural crest cells give rise to sympathetic neurons, most of which are noradrenergic. To determine if there is an intrinsic difference in the ability of cranial and trunk neural crest cells to form cholinergic neurons, we have compared the development of choline acetyltransferase (ChAT)-immunoreactive cells in explants of quail cranial and trunk neural crest in vitro. Both cranial and trunk neural crest explants gave rise to ChAT-immunoreactive cells in vitro. In both types of cultures, some of the ChAT-positive cells also expressed immunoreactivity for the catecholamine synthetic enzyme tyrosine hydroxylase. However, several differences were seen between cranial and trunk cultures. First, ChAT-immunoreactive cells appeared two days earlier in cranial than in trunk cultures. Second, cranial cultures contained a higher proportion of ChAT-immunoreactive cells. Finally, a subpopulation of the ChAT-immunoreactive cells in cranial cultures exhibited neuronal traits, including neurofilament immunoreactivity. In contrast, neurofilament-immunoreactive cells were not seen in trunk cultures. These results suggest that premigratory cranial and trunk neural crest cells differ in their ability to form cholinergic neurons.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(90)90257-JDOIArticle
http://www.sciencedirect.com/science/article/pii/001216069090257JPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne E.0000-0003-4274-1862
Additional Information:© 1990 Academic Press, Inc. Accepted 5 October 1989, Available online 4 November 2004. We thank Gregory Rafijah and Adela Augsberger for their excellent technical assistance. This work was supported by National Science Foundation Grant ENS 8702512 and a grant-in-aid from the American Heart Association, California Affiliate.
Funders:
Funding AgencyGrant Number
NSFENS 8702512
American Heart Association, California AffiliateUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160307-141031892
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160307-141031892
Official Citation:Differential development of cholinergic neurons from cranial and trunk neural crest cells in vitro Pages 318-330 Gabrielle G. Leblanc, Miles L. Epstein, Marianne E. Bronner-Frase
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65142
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:15 Mar 2016 01:21
Last Modified:03 Oct 2019 09:44

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