CaltechAUTHORS
  A Caltech Library Service

Low pH-dependent Sindbis virus-induced fusion of bhk cells: Differences between strains correlate with amino acid changes in the E1 glycoprotein

Boggs, William M. and Hahn, Chang S. and Strauss, Ellen G. and Strauss, James H. and Griffin, Diane E. (1989) Low pH-dependent Sindbis virus-induced fusion of bhk cells: Differences between strains correlate with amino acid changes in the E1 glycoprotein. Virology, 169 (2). pp. 485-488. ISSN 0042-6822. http://resolver.caltech.edu/CaltechAUTHORS:20160307-152609695

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20160307-152609695

Abstract

Expression of alphavirus glycoproteins on the surface of infected cells leads to cell fusion after exposure to acidic pH. Two strains of Sindbis virus, AR339 (SV) and neuroadapted Sindbis virus (NSV), which differ in virulence for weanling mice, were found to differ in pH-dependent fusion. BHK-21 cells infected with SV fused maximally after shifting to pH 5.4, whereas cells infected with NSV required a lower pH, pH 4.8, for maximal fusion. No difference was noted in the optimal pH for agglutination of goose erythrocytes (5.75 for both viruses). To determine the molecular basis for the difference in fusion a series of recombinant viruses was constructed using a cDNA clone of Sindbis virus from which infectious RNA can be transcribed in vitro. Cells infected with a recombinant virus that had the SV E1 and NSV E2 genes had a fusion response curve as a function of pH like SV, while cells infected with recombinant virus with the NSV E1 and SV E2 genes fused like NSV. The E1 glycoproteins of SV and NSV differ at two positions: Val-72 in SV is Ala in NSV (a change near the putative fusion site), and Gly-313 in SV is Asp in NSV. Recombinant viruses which had Val-72 (SV) and Asp-313 (NSV) or Ala-72 (NSV) and Gly-313 (SV) had a lowered pH of fusion like NSV suggesting that both positions participate in determining some aspect of the conformational change in the E1-E2 heterodimer associated with pH-dependent fusion.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0042-6822(89)90178-5DOIArticle
http://www.sciencedirect.com/science/article/pii/0042682289901785PublisherArticle
Additional Information:© 1989 Academic Press, Inc. Received September 27, 1988; accepted November 21, 1988. We thank John Humpal for excellent technical assistance and Linda Kelly for preparation of the manuscript. This work was supported by Public Health Services Grants NS 18596 (D.E.G.), AI 10793, and AI 20612 (J.H.S.) from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
NIHNS 18596
NIHAI 10793
NIHAI 20612
Record Number:CaltechAUTHORS:20160307-152609695
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160307-152609695
Official Citation:William M. Boggs, Chang S. Hahn, Ellen G. Strauss, James H. Strauss, Diane E. Griffin, Low pH-dependent sindbis virus-induced fusion of bhk cells: Differences between strains correlate with amino acid changes in the E1 glycoprotein, Virology, Volume 169, Issue 2, 1989, Pages 485-488, ISSN 0042-6822, http://dx.doi.org/10.1016/0042-6822(89)90178-5. (http://www.sciencedirect.com/science/article/pii/0042682289901785)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65155
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:08 Mar 2016 03:51
Last Modified:08 Mar 2016 03:52

Repository Staff Only: item control page