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Dideoxy Sequencing of RNA Using Reverse Transcriptase

Hahn, Chang S. and Strauss, Ellen G. and Strauss, James H. (1989) Dideoxy Sequencing of RNA Using Reverse Transcriptase. In: RNA Processing Part A: General Methods. Methods in Enzymology. No.180. Academic Press , pp. 121-130. ISBN 978-0-12-182081-7.

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Shortly after the dideoxy (dd) chain-termination method for DNA sequencing was described by Sanger et al., Zimmern and Kaesberg modified this method to sequence RNA directly, using purified encephalomyocarditis viral RNA as a template, pdT_(7)rC as a specific primer, and reverse transcriptase as the elongation enzyme. Bina-Stein et al. further developed the method to use total cellular mRNA as a template and complementary DNA restriction fragments, which anneal to a particular mRNA at specific locations, as primers for reverse transcriptase. Recently, sequence-specific oligonucleotides, synthesized with an automated DNA synthesizer, have been used as primers on both DNA and RNA templates.

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Additional Information:© 1989 Academic Press. Inc. This work was supported by Grants AI 10793 and AI 20612 from the National Institutes of Health and by Grant DMB 86-17372 from the National Science Foundation.
Funding AgencyGrant Number
NIHAI 10793
NIHAI 20612
NSFDMB 86-17372
Series Name:Methods in Enzymology
Issue or Number:180
Record Number:CaltechAUTHORS:20160307-152609982
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Official Citation:Chang Shahn, Ellen G. Strauss, James H. Strauss, [11] Dideoxy sequencing of RNA using reverse transcriptase, Methods in Enzymology, Academic Press, 1989, Volume 180, Pages 121-130, ISSN 0076-6879, ISBN 9780121820817, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65156
Deposited By: Donna Wrublewski
Deposited On:08 Mar 2016 04:00
Last Modified:10 Nov 2021 23:40

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