Hahn, Chang S. and Strauss, Ellen G. and Strauss, James H. (1989) Dideoxy Sequencing of RNA Using Reverse Transcriptase. In: RNA Processing Part A: General Methods. Methods in Enzymology. No.180. Academic Press , pp. 121-130. ISBN 978-0-12-182081-7. https://resolver.caltech.edu/CaltechAUTHORS:20160307-152609982
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160307-152609982
Abstract
Shortly after the dideoxy (dd) chain-termination method for DNA sequencing was described by Sanger et al., Zimmern and Kaesberg modified this method to sequence RNA directly, using purified encephalomyocarditis viral RNA as a template, pdT_(7)rC as a specific primer, and reverse transcriptase as the elongation enzyme. Bina-Stein et al. further developed the method to use total cellular mRNA as a template and complementary DNA restriction fragments, which anneal to a particular mRNA at specific locations, as primers for reverse transcriptase. Recently, sequence-specific oligonucleotides, synthesized with an automated DNA synthesizer, have been used as primers on both DNA and RNA templates.
| Item Type: | Book Section | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| |||||||||
| Additional Information: | © 1989 Academic Press. Inc. This work was supported by Grants AI 10793 and AI 20612 from the National Institutes of Health and by Grant DMB 86-17372 from the National Science Foundation. | |||||||||
| Funders: |
| |||||||||
| Series Name: | Methods in Enzymology | |||||||||
| Issue or Number: | 180 | |||||||||
| DOI: | 10.1016/0076-6879(89)80097-7 | |||||||||
| Record Number: | CaltechAUTHORS:20160307-152609982 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160307-152609982 | |||||||||
| Official Citation: | Chang Shahn, Ellen G. Strauss, James H. Strauss, [11] Dideoxy sequencing of RNA using reverse transcriptase, Methods in Enzymology, Academic Press, 1989, Volume 180, Pages 121-130, ISSN 0076-6879, ISBN 9780121820817, http://dx.doi.org/10.1016/0076-6879(89)80097-7. (http://www.sciencedirect.com/science/article/pii/0076687989800977) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 65156 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Donna Wrublewski | |||||||||
| Deposited On: | 08 Mar 2016 04:00 | |||||||||
| Last Modified: | 10 Nov 2021 23:40 |
Repository Staff Only: item control page
