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Effects of Antibodies against N-Cadherin and N-CAM on the Cranial Neural Crest and Neural Tube

Bronner-Fraser, Marianne and Wolf, John J. and Murray, Ben A. (1992) Effects of Antibodies against N-Cadherin and N-CAM on the Cranial Neural Crest and Neural Tube. Developmental Biology, 153 (2). pp. 291-301. ISSN 0012-1606. doi:10.1016/0012-1606(92)90114-V.

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We have examined the distribution and function of the defined cell adhesion molecules, N-cadherin and N-CAM, in the emigration of cranial neural crest cells from the neural tube in vivo. By immunocytochemical analysis, both N-cadherin and N-CAM were detected on the cranial neural folds prior to neural tube closure. After closure of the neural tube, presumptive cranial neural crest cells within the dorsal aspect of the neural tube had bright N-CAM and weak N-cadherin immunoreactivity. By the 10- to 11-somite stage, N-cadherin was prominent on all neural tube cells with the exception of the dorsal-most cells, which had little or no detectable immunoreactivity. N-CAM, but not N-cadherin, was observed on some migrating neural crest cells after their departure from the cranial neural tube. To examine the functional significance of these molecules, perturbation experiments were performed by injecting antibodies against N-CAM or N-cadherin into the cranial mesenchyme adjacent to the midbrain. Fab' fragments or whole IgGs of monoclonal and polyclonal antibodies against N-CAM caused abnormalities in the cranial neural tube and neural crest. Predominantly observed defects included neural crest cells in ectopic locations, both within and external to the neural tube, and mildly deformed neural tubes containing some dissociating cells. A monoclonal antibody against N-cadherin also disrupted cranial development, with the major defect being grossly distorted neural tubes and some ectopic neural crest cells outside of the neural tube. In contrast, nonblocking N-CAM antibodies and control IgGs had few effects. Embryos appeared to be sensitive to the N-CAM and N-cadherin antibodies for a limited developmental period from the neural fold to the 9-somite stage, with older embryos no longer displaying defects after antibody injection. These results suggest that the cell adhesion molecules N-CAM and N-cadherin are important for the normal integrity of the cranial neural tube and for the emigration of neural crest cells. Because cell-matrix interactions also are required for proper emigration of cranial neural crest cells, the results suggest that the balance between cell-cell and cell-matrix adhesion may be critical for this process.

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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1992 Academic Press, Inc. Accepted 8 May 1992. We thank Drs. Masatoshi Takeichi, Jack Lillien, and David Gottlieb for kindly providing antibodies used in this study and Brad Martinsen for excellent technical assistance. This work was supported by USPHS Grant DE10066 to M.B.-F. and by American Cancer Society Grant CD-416 to B.A.M.
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American Cancer SocietyCD-416
Issue or Number:2
Record Number:CaltechAUTHORS:20160308-065959357
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Official Citation:Marianne Bronner-Fraser, John J. Wolf, Ben A. Murray, Effects of antibodies against N-cadherin and N-CAM on the cranial neural crest and neural tube, Developmental Biology, Volume 153, Issue 2, October 1992, Pages 291-301, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65168
Deposited By: Ruth Sustaita
Deposited On:10 Mar 2016 23:24
Last Modified:10 Nov 2021 23:41

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