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Development of Cholinergic Traits in the Quail Ciliary Ganglion: Expression of Choline Acetyltransferase-like Immunoreactivity

Coulombe, J. and Bronner-Fraser, M. (1990) Development of Cholinergic Traits in the Quail Ciliary Ganglion: Expression of Choline Acetyltransferase-like Immunoreactivity. Neuroscience, 37 (1). pp. 259-270. ISSN 0306-4522. https://resolver.caltech.edu/CaltechAUTHORS:20160308-072342329

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Abstract

The avian ciliary ganglion is a parasympathetic ganglion derived from the neurol crest whose neurons provide cholinergic innervation to the eye. Here, we describe the time course of appearance and the morphology of cholinergic cells in the ciliary ganglion, as assessed by antibodies against choline acetyltransferase. Choline acetyltransferase immunoreactivity was first observed in 5.5-day-old quail embryos, 1 day after condensation of the ciliary ganglion. Both the intensity of choline acetyltransferase immunoreactivity and size of the choline acetyltransferase-immunoreactive cells increased with ganglionic age. By 12 days, a second population of choline acetyltransferase-immunoreactive cells, possibly corresponding to choroid neurons, was observed whose cells were smaller and less intensely stained than earlier differentiating choline acetyltransferase-immunoreactive cells. The percentage of choline acetyltransferase-immunoreactive cells was initially high, constituting approximately 50% of the total cell population. As a function of time, the proportion of cholinergic cells decreased, probably due to proliferation of non-neuronal cells and naturally-occurring cell death. Our results confirm the existence of two morphologically distinct populations of cholinergic neurons in the avian ciliary ganglion and demonstrate that these neuronal subpopulations express choline acetyltransferase immunoreactivity at different times in development. Because choroid neurons innervate their targets later than ciliary neurons, this finding is consistent with the hypothesis that target interactions regulate expression of choline acetyltransferase.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0306-4522(90)90212-MDOIArticle
http://www.sciencedirect.com/science/article/pii/030645229090212MPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, M.0000-0003-4274-1862
Additional Information:© 1990 Pergamon Press Inc. Accepted 27 November 1989. We thank Dr Gabrielle Leblanc for her critical reading of the manuscript and helpful suggestions. We are also indebted to Drs Miles Epstein and Paul Salvaterra for generous gifts of antibody and purified choline acetyltransferase. This work was supported by DCB 85-02604 and BNS 86-07760. M.B.F. is a Sloan Foundation Fellow.
Funders:
Funding AgencyGrant Number
NSFDCB 85-02604
NSFBNS 86-07760
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20160308-072342329
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160308-072342329
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65171
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:08 Mar 2016 16:51
Last Modified:03 Oct 2019 09:44

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