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Membrane-Associated Neurotransmitter Stimulating Factor is Very Similar to Ciliary Neurotrophic Factor

Rao, M. and Patterson, P. H. and Landis, S. C. (1992) Membrane-Associated Neurotransmitter Stimulating Factor is Very Similar to Ciliary Neurotrophic Factor. Developmental Biology, 153 (2). pp. 411-416. ISSN 0012-1606. doi:10.1016/0012-1606(92)90127-3.

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Membrane-associated neurotransmitter stimulating factor (MANS) can modulate sympathetic neurotransmitter expression and promote ciliary neuron survival in cell culture. Previous studies have shown that its biological effects and biochemical properties are similar to those of ciliary neurotrophic factor (CNTF). In addition, CNTF is present in spinal cord, the source of MANS. These observations raised the possibility that MANS preparations contain CNTF. We find that partially purified MANS fractions contain a 24-kD protein that is recognized in Western blots by an antiserum generated against recombinant rat CNTF (rCNTF). This antiserum immunoprecipitates virtually all the cholinergic-inducing and the ciliary neurotrophic activities present in MANS preparations. When iodinated rCNTF is incubated with spinal cord membranes, a significant proportion of the labeled CNTF segregates with the membrane pellet. The membrane-associated exogenous CNTF can be eluted from the membrane fraction by treatment with high-salt solutions, similar to that used to solubilize MANS from spinal cord membranes. Our data suggest that a substantial portion of the cholinergic differentiation and ciliary neurotrophic activities present in MANS preparations can be attributed to CNTF or a CNTF-like molecule.

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Additional Information:© 1992 Academic Press, Inc. Accepted 27 May 1992. The authors thank Dr. Keet for providing NGF, Dr. Morrisey and Dr. Furth of Regeneron Pharmaceuticals for recombinant CNTF and anti-CNTF antiserum, and Dr. Samal of Amgen Pharmaceuticals for recombinant human LIF. This work was supported by NINDS Javits Neuroscience Investigator awards to P.H.P. and S.C.L., a McKnight Foundation Neuroscience Research Project Award to P.H.P., and an American Heart Association Grant-in-Aid to S.C.L. M.R. is a recipient of a postdoctoral fellowship from the Northeast Ohio Affiliate of the American Heart Association.
Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
McKnight FoundationUNSPECIFIED
American Heart Association, Northeast Ohio AffiliateUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160308-081259465
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Official Citation:M. Rao, P.H. Patterson, S.C. Landis, Membrane-associated neurotransmitter stimulating factor is very similar to ciliary neurotrophic factor, Developmental Biology, Volume 153, Issue 2, October 1992, Pages 411-416, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65176
Deposited By: Ruth Sustaita
Deposited On:08 Mar 2016 17:57
Last Modified:10 Nov 2021 23:41

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