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Separation and Partial Characterization of Neuropeptide-lnducing Factors in Heart Cell Conditioned Medium

Nawa, Hiroyuki and Patterson, Paul H. (1990) Separation and Partial Characterization of Neuropeptide-lnducing Factors in Heart Cell Conditioned Medium. Neuron, 4 (2). pp. 269-277. ISSN 0896-6273. https://resolver.caltech.edu/CaltechAUTHORS:20160308-082814234

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Abstract

Various conditioned media contain multiple factors that regulate the expression of the neurotransmitters acetylcholine, serotonin, and catecholamines and the neuropeptides substance P, somatostatin, vasoactive intestinal polypeptide-related peptides, cholecystokinin, and enkephalins in cultured sympathetic neurons. Using biochemical and immunological methods, we identify at least three distinct factors in heart cell conditioned medium: one induces acetylcholine, substance P, somatostatin, and vasoactive intestinal polypeptide-related peptides while suppressing catecholamine expression, a second factor induces only vasoactive intestinal polypeptide-related peptides, and a third factor induces only somatostatin expression. These observations demonstrate the existence of a group of biochemically and immunologically distinct factors involved in phenotypic specification with unique, but partially overlapping activities. The analogy with the family of differentiation factors in the hematopoietic system is discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0896-6273(90)90101-KDOIArticle
http://www.sciencedirect.com/science/article/pii/089662739090101KPublisherArticle
Additional Information:© 1990 Cell Press. Received 1 September 1989, Revised 2 November 1989. We wish to thank Keiko Fukada for helpful discussions and detailed advice about protein purification, Bruce Patton for instruction concerning HPLC, Sigrun Korsching and Mahendra Rao for providing affinity-purified anti-heart cell cholinergic factor antiserum, and Nagesh Mahanthappa and Dinah Sah for comments on the manuscript. We are also grateful to Doreen McDowell for the preparation of tissue culture media and collagen, and Josette Carnahan for the preparation of nerve growth factor. This work was supported by a Javits Neuroscience Investigator Award and a McKnight Foundation Neuroscience Research Project Award to P. H. P. H. N. was a postdoctoral fellow of the Muscular Dystrophy Association and the Del Webb Foundation.
Funders:
Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
McKnight FoundationUNSPECIFIED
Muscular Dystrophy AssociationUNSPECIFIED
Del Webb FoundationUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160308-082814234
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160308-082814234
Official Citation:Hiroyuki Nawa, Paul H. Patterson, Separation and partial characterization of neuropeptide-inducing factors in heart cell conditioned medium, Neuron, Volume 4, Issue 2, February 1990, Pages 269-277, ISSN 0896-6273, http://dx.doi.org/10.1016/0896-6273(90)90101-K. (http://www.sciencedirect.com/science/article/pii/089662739090101K)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65179
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:08 Mar 2016 17:59
Last Modified:03 Oct 2019 09:44

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