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A Monoclonal Antibody That Defines Rostrocaudal Gradients in the Mammalian Nervous System

Suzue, Toshihiko and Kaprielian, Zaven and Patterson, Paul H. (1990) A Monoclonal Antibody That Defines Rostrocaudal Gradients in the Mammalian Nervous System. Neuron, 5 (4). pp. 421-431. ISSN 0896-6273. https://resolver.caltech.edu/CaltechAUTHORS:20160308-083706291

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Abstract

Spinal cord axons display a rostrocaudal, positional bias in their innervation of sympathetic ganglia and inter-costal skeletal muscles. In an effort to examine the molecular basis of this positional specificity, we used the cyclophosphamide immunosuppression method to produce monoclonal antibodies that bind preferentially to rostral ganglia. The staining distribution of one of these antibodies, ROCA1, has been analyzed using a novel histological method. A graded decline in binding is observed along the chain of adult rat sympathetic ganglia, as well as in the nerves innervating intercostal muscles. The antigen is identified on immunoblots as a 65 kd protein, whose distribution corresponds to the pattern found histologically. Surprisingly, ROCA1 appears to bind to glial cells, implying rostrocaudal, molecular differences in their surfaces.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0896-6273(90)90081-PDOIArticle
http://www.sciencedirect.com/science/article/pii/089662739090081PPublisherArticle
Additional Information:© 1990 Cell Press. Received 7 June 1990, Revised 20 July 1990. We thank Charles Yee for considerable help with the histology and antibodies, Joann lmrich, Bill Matthew, Josh Sanes, and Jon Covault for advice, and Arlene Chiu, David Anderson, and members of our group for reading the manuscript. This project was supported by a Developmental Biology Grant from the Lucille P. Markey Charitable Trust and a McKnight Foundation Neuroscience Research Project Award to P.H. P., a Muscular Dystrophy Association fellowship to Z. K., and a Japanese Ministry of Education, Science and Culture fellowship to T. S. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Lucille P. Markey Charitable TrustUNSPECIFIED
McKnight FoundationUNSPECIFIED
Muscular Dystrophy AssociationUNSPECIFIED
Ministry of Education, Science and Culture (Japan)UNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20160308-083706291
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160308-083706291
Official Citation:Toshihiko Suzue, Zaven Kaprielian, Paul H. Patterson, A monoclonal antibody that defines rostrocaudal gradients in the mammalian nervous system, Neuron, Volume 5, Issue 4, October 1990, Pages 421-431, ISSN 0896-6273, http://dx.doi.org/10.1016/0896-6273(90)90081-P. (http://www.sciencedirect.com/science/article/pii/089662739090081P)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65180
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:08 Mar 2016 21:54
Last Modified:03 Oct 2019 09:44

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