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Ruthenium complexes as luminescent reporters of DNA

Murphy, Catherine J. and Barton, Jacqueline K. (1993) Ruthenium complexes as luminescent reporters of DNA. In: Metallobiochemistry Part C: Spectroscopic and Physical Methods for Probing Metal Ion Environments in Metalloenzymes and Metalloproteins. Methods in Enzymology. No.226. Academic Press , San Diego, CA, pp. 576-594. ISBN 9780121821272.

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Coordination chemistry provides a wealth of characteristics that may be exploited in probing nucleic acids. Structurally and stereochemically well-defined transition metal complexes have been designed to probe nucleic acid structure and the recognition process. Reactive transition metal complexes have been tethered onto DNA-binding moieties to report sensitively on their recognition characteristics. This chapter discusses coordination complexes that have been remarkably useful as spectroscopic tags. In particular, it describes the application of ruthenium complexes to probe nucleic acid structure and recognition. Polypyridyl complexes of ruthenium(II) and their derivatives possess several features that may be exploited in developing spectroscopic probes for nucleic acids. The complexes possess an intense metal-toligand charge transfer (MLCT) transition in the visible region which yields a luminscent excited state; the excited state characteristics of these complexes have been amply characterized. Most importantly, this transition is perturbed on binding to DNA. The complexes are coordinatively saturated. They are inert to substitution and are stable in aqueous solution. Because the complexes are octahedral and possess three bidentate ligands, the complexes are chiral.

Item Type:Book Section
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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 1993 Academic Press, Inc. We are grateful to the National Institute of General Medical Science (GM33309) and to the National Science Foundation (postdoctoral fellowship to C.J.M.) for financial support. We are also grateful to our co-workers and collaborators, as described in the individual references, for scientific contributions in developing the methodology described here. Essential to the development of this research has also been the insights of our long-time collaborator, Nicholas J. Turro.
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NSF Postdoctoral FellowshipUNSPECIFIED
Series Name:Methods in Enzymology
Issue or Number:226
Record Number:CaltechAUTHORS:20160308-093657057
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Official Citation:Catherine J. Murphy, Jacqueline K. Barton, [25] Ruthenium complexes as luminescent reporters of DNA, Methods in Enzymology, Academic Press, 1993, Volume 226, Pages 576-594, ISSN 0076-6879, ISBN 9780121821272, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65185
Deposited By: Ruth Sustaita
Deposited On:09 Mar 2016 23:49
Last Modified:03 Oct 2019 09:44

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