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Neuronal expression of leukemia inhibitory factor (LIF) in the rat brain

Lemke, R. and Gadient, R. A. and Schliebs, R. and Bigl, V. and Patterson, P. H. (1996) Neuronal expression of leukemia inhibitory factor (LIF) in the rat brain. Neuroscience Letters, 215 (3). pp. 205-208. ISSN 0304-3940. doi:10.1016/0304-3940(96)12986-4.

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Very little is known about the action of the leukemia inhibitory factor (LIF) in the central nervous system. To analyze LIF expression in adult rat brain, we used non-radioactive in situ hybridization with digoxigenin-labeled RNA probes, in combination with immunohistochemistry to identify the cell types expressing LIF mRNA. Hippocampal pyramidal cells, hilar interneurons and granule cells of the dentate gyrus display strong hybridization signals, while neurons of the cerebral cortex, cerebellum, cholinergic basal forebrain nuclei, and the striatum are labeled as well. The most prominent expression in cerebral cortex is in layers IV and VI. Glial fibrillary acidic protein-positive astrocytes did not display a detectable LIF hybridization signal suggesting that LIF mRNA is predominantly expressed in neurons in the normal, adult rat brain, although a presence in resting microglial cells cannot be excluded.

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Additional Information:© 1996 Elsevier Ireland Ltd. Received 8 July 1996; revised version received 14 August 1996; accepted 14 August 1996. This work was supported by a grant of the NIH to P.H.P and by the Bundesministerium für Forschung und Technik, grant FKZ 01 ZZ 9130 to R.S.R.L. is a recipient of a stipend from the Studienstiftung des Deutschen Volkes e.V., and R.A.G. is supported by the Swiss National Science Foundation and the Roche Research Foundation. In addition, we gratefully acknowledge the University of Leipzig Medical School, Germany for covering travel expenses, and Dr. H. Govan for critical comments.
Funding AgencyGrant Number
Bundesministerium für Forschung und TechnikFKZ 01 ZZ 9130
Studienstiftung des Deutschen VolkesUNSPECIFIED
Swiss National Science Foundation (SNSF)UNSPECIFIED
Roche Research FoundationUNSPECIFIED
University of LeipzigUNSPECIFIED
Subject Keywords:Cholinergic differentiation factor; Glial fibrillary acidic protein; Rat brain; Ciliary neurotrophic factor; Interleukin-6
Issue or Number:3
Record Number:CaltechAUTHORS:20160308-135514574
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Official Citation:R. Lemke, R.A. Gadient, R. Schliebs, V. Bigl, P.H. Patterson, Neuronal expression of leukemia inhibitory factor (LIF) in the rat brain, Neuroscience Letters, Volume 215, Issue 3, 13 September 1996, Pages 205-208, ISSN 0304-3940, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65213
Deposited By: Tony Diaz
Deposited On:10 Mar 2016 21:08
Last Modified:10 Nov 2021 23:41

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