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Snail-Related Transcriptional Repressors Are Required in Xenopus for both the Induction of the Neural Crest and Its Subsequent Migration

LaBonne, C. and Bronner-Fraser, M. (2000) Snail-Related Transcriptional Repressors Are Required in Xenopus for both the Induction of the Neural Crest and Its Subsequent Migration. Developmental Biology, 221 (1). pp. 195-205. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160310-123654012

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Abstract

The neural crest is a transient population of precursor cells that arises at the border between the neural plate and prospective epidermis in vertebrate embryos. The earliest known response to neural-crest-inducing signals is the expression of the zinc-finger transcription factors slug and snail. Although it is widely believed that these transcription factors play an essential role in neural crest development, relatively little is understood about their mechanism of action during this process. We have previously shown that overexpression of XSlug leads to expanded expression of neural crest markers and an excess of at least one neural crest derivative, melanocytes. In order to further investigate XSlug function, we overexpressed mutant constructs in which the DNA-binding domain was fused to either the activation domain from Gal4 or the repressor domain from Drosophila Engrailed. The Engrailed repressor fusion was found to mimic the effects of wild-type XSlug, indicating that XSlug functions as a transcriptional repressor during neural crest formation. In contrast, overexpression of either the activation domain fusion or the DNA-binding domain alone was found to inhibit XSlug function. Using a hormone-inducible inhibitory mutant, we show that inhibition of XSlug function at early stages prevents the formation of neural crest precursors, while inhibition at later stages interferes with neural crest migration, demonstrating for the first time that this transcriptional repressor is required during multiple stages of neural crest development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2000.9609DOIArticle
http://www.sciencedirect.com/science/article/pii/S001216060099609XPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, M.0000-0003-4274-1862
Additional Information:© 2000 Academic Press. Received 22 October 1999, Revised 20 December 1999, Accepted 20 December 1999. We thank Clare Baker, Ann Knecht, and David McCauley for critical reading of the manuscript and Adrian Gross, Laura Gammill, and members of the laboratory for helpful discussions. pCS21 derivatives were kindly provided by Robert Davis and Dave Turner. C.L. is a fellow of the American Cancer Society. This work was also supported by USPHS NS36585 and NS34671 to M.B-F.
Funders:
Funding AgencyGrant Number
American Cancer SocietyUNSPECIFIED
NIHNS36585
NIHNS34671
Subject Keywords:neural crest; slug; snail; Xenopus
Issue or Number:1
Record Number:CaltechAUTHORS:20160310-123654012
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160310-123654012
Official Citation:C. LaBonne, M. Bronner-Fraser, Snail-Related Transcriptional Repressors Are Required in Xenopus for both the Induction of the Neural Crest and Its Subsequent Migration, Developmental Biology, Volume 221, Issue 1, 1 May 2000, Pages 195-205, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.2000.9609. (http://www.sciencedirect.com/science/article/pii/S001216060099609X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65262
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Mar 2016 20:55
Last Modified:03 Oct 2019 09:45

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