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Receptor-targeted co-transport of DNA and magnetic resonance contrast agents

Kayyem, Jon Faiz and Kumar, Roshan M. and Fraser, Scott E. and Meade, Thomas J. (1995) Receptor-targeted co-transport of DNA and magnetic resonance contrast agents. Chemistry and Biology, 2 (9). pp. 615-620. ISSN 1074-5521. https://resolver.caltech.edu/CaltechAUTHORS:20160310-135832264

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Abstract

Background: Ligand molecules conjugated to polylysine can be electrostatically bound to DNA and can bind receptors or antigens on the surface of cells, delivering the DNA into specific cells and tissues. Several researchers have used this approach to generate non-viral vehicles for the efficient delivery of DNA to specific cells. We have attempted to adopt this general approach to the cell-specific delivery of magnetic contrast agents for use in magnetic resonance imaging (MRI). Results: We have synthesized a new class of agents capable of both transfecting genes into cells and enhancing the contrast of the targeted cells for MRI. DNA is used both to encode a marker gene and as a molecular scaffold, which electrostatically binds polylysine conjugated to transferrin, an iron uptake protein, and polylysine modified with gadolinium chelated to diethylenetriaminepetaacetic acid. When cells displaying the transferrin receptor are treated with these particles, high levels of gene expression are observed, higher than with control particles composed only of transferrin, polylysine and DNA. The treated cells show specific MRI contrast enhancement, which did not require expression of the marker gene. Conclusions: The development of this class of particles permits the use of novel protocols by which genes for genetic therapy and agents for MRI contrast are cotransported. These protocols may allow non-invasive MRI monitoring of DNA delivery for gene therapy in real time.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/1074-5521(95)90126-4DOIArticle
http://www.sciencedirect.com/science/article/pii/1074552195901264PublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 1995 Elsevier Ltd. Received: 4 Aug 1995; revisions requested: 22 Aug 1995; revisions received: 1 Sep 1995. Accepted: 5 Sep 1995. The authors thank the Baxter Foundation for generous support of this work. We thank Pratik Gosh and Rex Moats for assistance with image acquisition and Kim Mislick for helpful discussions. R.M.K. was supported in part by a Summer Undergraduate Research Fellowship at Caltech.
Funders:
Funding AgencyGrant Number
Baxter FoundationUNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
Subject Keywords:contrast agent; gene delivery; MR[; polylysine; transferrin
Issue or Number:9
Record Number:CaltechAUTHORS:20160310-135832264
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160310-135832264
Official Citation:Jon Faiz Kayyem, Roshan M. Kumar, Scott E. Fraser, Thomas J. Meade, Receptor-targeted co-transport of DNA and magnetic resonance contrast agents, Chemistry & Biology, Volume 2, Issue 9, September 1995, Pages 615-620, ISSN 1074-5521, http://dx.doi.org/10.1016/1074-5521(95)90126-4. (http://www.sciencedirect.com/science/article/pii/1074552195901264)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65265
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Mar 2016 22:23
Last Modified:03 Oct 2019 09:45

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