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SpGCF1, a Sea Urchin Embryo DNA-Binding Protein, Exists as Five Nested Variants Encoded by a Single mRNA

Zeller, Robert W. and Coffman, James A. and Harrington, Michael G. and Britten, Roy J. and Davidson, Eric H. (1995) SpGCF1, a Sea Urchin Embryo DNA-Binding Protein, Exists as Five Nested Variants Encoded by a Single mRNA. Developmental Biology, 169 (2). pp. 713-727. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160311-071745501

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Abstract

Several Strongylocentrotus purpuratus gene cis-regulatory regions contain asymmetric C_4 sequences which are core elements of target sites for a specific DNA-protein interaction. Blastula stage nuclear extract contains five proteins which specifically bind to these target sites, resulting in a characteristic pattern of complexes in gel mobility shift assays. We used automated affinity chromatography to purify a protein which binds to these sites and have isolated the corresponding cDNA. This protein, SpGCF1, is a novel sea urchin DNA-binding protein with no overall homology to proteins reported in the databases currently available. The DNA-binding domain of this protein was identified by a deletion analysis. As demonstrated both for protein translated in vitro and for bacterial protein expressed from a cDNA clone, a single SpGCF1 mRNA serves as a template for the synthesis of five DNA-binding polypeptides. We show that these five polypeptides are most likely produced by differential usage of a nested set of AUG start codons in the SpGCF1 cDNA and thus contain variable amounts of a proline-rich N-terminal domain. Since proline-rich regions often serve as transcriptional activation domains, the five SpGCF1 proteins apparently possess different "activation potentials."


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1995.1181DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160685711815PublisherArticle
ORCID:
AuthorORCID
Harrington, Michael G.0000-0002-7923-8032
Additional Information:© 1995 Academic Press, Inc. Accepted March 17, 1995. We are grateful to Professors Carl Parker and Scott Fraser of this Institute for critical reviews of the manuscript. This research was supported by NIH Grant HD-05753. R.W.Z. was an AASERT awardee of the ONR.
Funders:
Funding AgencyGrant Number
NIHHD-05753
Office of Naval Research (ONR)UNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160311-071745501
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160311-071745501
Official Citation:Robert W. Zeller, James A. Coffman, Michael G. Harrington, Roy J. Britten, Eric H. Davidson, SpGCF1, a Sea Urchin Embryo DNA-Binding Protein, Exists as Five Nested Variants Encoded by a Single mRNA, Developmental Biology, Volume 169, Issue 2, June 1995, Pages 713-727, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.1995.1181. (http://www.sciencedirect.com/science/article/pii/S0012160685711815)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65290
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:14 Mar 2016 20:32
Last Modified:03 Oct 2019 09:45

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