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Architecture of the type IVa pilus machine

Chang, Yi-Wei and Rettberg, Lee A. and Treuner-Lange, Anke and Iwasa, Janet and Søgaard-Andersen, Lotte and Jensen, Grant J. (2016) Architecture of the type IVa pilus machine. Science, 351 (6278). aad2001. ISSN 0036-8075. PMCID PMC5929464. https://resolver.caltech.edu/CaltechAUTHORS:20160315-090327567

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[img] PDF (Materials and Methods; Figs. S1 to S14; Tables S1 to S5; Captions for movies S1 to S3; References) - Supplemental Material
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[img] Video (QuickTime) (Movie S1 - Cryo-EM tilt series of a M. xanthus cell pole) - Supplemental Material
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[img] Video (QuickTime) (Movie S2 - Cryotomogram of a M. xanthus cell pole capturing T4PM structures) - Supplemental Material
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[img] Video (QuickTime) (Movie S3 - Comparison of the T4PM conformation in piliated and empty states) - Supplemental Material
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Abstract

Many bacteria, including important pathogens, move by projecting grappling-hook–like extensions called type IV pili from their cell bodies. After these pili attach to other cells or objects in their environment, the bacteria retract the pili to pull themselves forward. Chang et al. used electron cryotomography of intact cells to image the protein machines that extend and retract the pili, revealing where each protein component resides. Putting the known structures of the individual proteins in place like pieces of a three-dimensional puzzle revealed insights into how the machine works, including evidence that ATP hydrolysis by cytoplasmic motors rotates a membrane-embedded adaptor that slips pilin subunits back and forth from the membrane onto the pilus.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.aad2001DOIArticle
http://www.sciencemag.org/content/351/6278/aad2001/suppl/DC1PublisherSupplementary Materials
http://dx.doi.org/10.1126/science.aaf7977DOIErratum
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929464PubMed CentralArticle
https://doi.org/10.1038/nrmicro.2016.51Featured InNature Reviews Microbiology - Research Highlights
ORCID:
AuthorORCID
Chang, Yi-Wei0000-0003-2391-473X
Rettberg, Lee A.0000-0002-5366-1609
Treuner-Lange, Anke0000-0001-6435-5642
Iwasa, Janet0000-0002-4949-7607
Søgaard-Andersen, Lotte0000-0002-0674-0013
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2016 American Association for the Advancement of Science. Received 10 August 2015; accepted 13 January 2016. We thank C. Oikonomou and D. Ortega for discussions. Supported by NIH grant R01 GM094800B (G.J.J.), the Howard Hughes Medical Institute, the Max Planck Society, and the Deutsche Forschungsgemeinschaft within the framework of the Collaborative Research Center (SFB) 987 “Microbial Diversity in Environmental Signal Response.” The 18 subtomogram averages of T4PMs reported in this study have been deposited in the Electron Microscopy Data Bank with accession numbers EMD-3247 (wild type, piliated); EMD-3248 (wild type, empty); EMD-3249 (ΔpilP, empty); EMD-3250 (PilP-sfGFP, piliated); EMD-3251 (PilP-sfGFP, empty); EMD-3252 (ΔtsaP, piliated); EMD-3253 (ΔtsaP, empty); EMD-3254 (PilO-sfGFP, piliated); EMD-3255 (PilO-sfGFP, empty); EMD-3256 (ΔpilC, empty); EMD-3257 (ΔpilA, empty); EMD-3258 (ΔpilV pilW fimU 1+2+3, empty); EMD-3259 (ΔpilT, piliated); EMD-3260 (ΔpilB, empty); EMD-3261 (ΔpilB ΔpilT, empty); EMD-3262 (ΔpilM, empty); EMD-3263 (ΔpilQβ1-β2, piliated); and EMD-3264 (ΔpilQβ1-β2, empty). The coordinates of the hypothetical T4PM working models have been deposited in the Protein Data Bank with accession numbers 3JC8 (piliated) and 3JC9 (empty), respectively. Author contributions: Y.-W.C. collected the cryo–electron tomography data, which were analyzed by Y.-W.C. and L.A.R.; Y.-W.C. built the T4PM models and generated the movie describing the modeling process; A.T.-L. and L.S.-A. provided the M. xanthus strains and characterized their motility and T4PM component accumulation and localization; J.I. produced the animation of T4PM dynamics; and Y.-W.C., L.S.-A., and G.J.J. wrote the paper.
Funders:
Funding AgencyGrant Number
NIHR01 GM094800B
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Max Planck SocietyUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Issue or Number:6278
PubMed Central ID:PMC5929464
Record Number:CaltechAUTHORS:20160315-090327567
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160315-090327567
Official Citation:Architecture of the type IVa pilus machine BY YI-WEI CHANG, LEE A. RETTBERG, ANKE TREUNER-LANGE, JANET IWASA, LOTTE SØGAARD-ANDERSEN, GRANT J. JENSEN SCIENCE 11 MAR 2016 Vol. 351, Issue 6278, DOI: 10.1126/science.aad2001
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65350
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Mar 2016 16:38
Last Modified:05 May 2022 18:32

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