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The in Vivo Expression of the FGF Receptor FREK mRNA in Avian Myoblasts Suggests a Role in Muscle Growth and Differentiation

Marcelle, Christophe and Wolf, John and Bronner-Fraser, Marianne (1995) The in Vivo Expression of the FGF Receptor FREK mRNA in Avian Myoblasts Suggests a Role in Muscle Growth and Differentiation. Developmental Biology, 172 (1). pp. 100-114. ISSN 0012-1606. doi:10.1006/dbio.1995.0008.

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Muscle growth during embryogenesis is the result of a balance between the proliferation of myoblasts and their differentiation into mature, contractile fibers. Fibroblast growth factors (FGFs) are potent stimulators of myoblast proliferation and inhibitors of myoblast differentiationin vitro.However, it is not clear at present if FGFs and their receptors regulate this processin vivo,partially because no FGF receptor was known to be expressed by myoblasts during embryogenesis. In this study, we have used quail/chick grafting and BrdU labeling techniques to demonstrate that a recently cloned avian FGF receptor, FREK, is expressed by replicating skeletal muscle myoblasts, while differentiated muscle cells no longer express this receptor. In the limb, muscle progenitors originating from the somite start expressing FREK at 3 days of development (E3). FREK expression in the limb myoblasts follows that of Pax-3 and Pax-7, but precedes that of MyoD. Since MyoD expression signals the onset of terminal differentiation, this demonstrates that FREK is expressed in muscle progenitors prior to overt muscle differentiation. A more complex situation is observed in the trunk region, where a first wave of MyoD-positive myocytes, which are postmitotic and never express FREK, appear in the early myotomal compartment of the somite. Slightly later, at E2.5, FREK-positive myoblasts migrate into the myotome as a second wave of muscle progenitors, 15 hr after the first MyoD-positive cells. FREK's expression by myoblasts arising at all stages of myogenesis indicates that this growth factor receptor represents one of the earliest molecular markers for this cell population. FREK's prominent expression during muscle differentiation sets it apart from other FGF receptors and suggests that this molecule plays an important role during muscle growth and differentiation.

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Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1995 Academic Press, Inc. Accepted June 14, 1995. We thank Dr. Charles Ordahl for discussion and helpful commments on the manuscript. We thank Dr. K. Tosney for insighful discussions during the course of this study. Chritophe Marcelle is a fellow of the Human Frontier Science Program Organization. This work was supported by HD 15527 and a grant from Muscular Dystrophy Association.
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U.S. Public Health Service (USPHS)HD-15527
Muscular Dystrophy AssociationUNSPECIFIED
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Official Citation:Christophe Marcelle, John Wolf, Marianne Bronner-Fraser, Thein VivoExpression of the FGF Receptor FREK mRNA in Avian Myoblasts Suggests a Role in Muscle Growth and Differentiation, Developmental Biology, Volume 172, Issue 1, November 1995, Pages 100-114, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65408
Deposited By: Ruth Sustaita
Deposited On:17 Mar 2016 20:05
Last Modified:10 Nov 2021 23:45

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