CaltechAUTHORS
  A Caltech Library Service

Effect of leukemia inhibitory factor (LIF) on the morphology and survival of cultured hippocampal neurons and glial cells

Gadient, Reto A. and Lein, Pamela and Higgins, Dennis and Patterson, Paul H. (1998) Effect of leukemia inhibitory factor (LIF) on the morphology and survival of cultured hippocampal neurons and glial cells. Brain Research, 798 (1-2). pp. 140-146. ISSN 0006-8993. https://resolver.caltech.edu/CaltechAUTHORS:20160317-081243333

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160317-081243333

Abstract

Leukemia inhibitory factor (LIF) is a cytokine involved in the survival, differentiation and regeneration of sympathetic, sensory and motor neurons. Its effects in the brain are less well characterized. In a previous study, we found LIF transcripts to be predominantly expressed in neurons of the adult rat brain. Highest levels were observed in the hippocampus, particularly in granular neurons of the dentate gyrus and in hilar interneurons. Here we report the effects of LIF on survival and differentiation of postnatal rat hippocampal cells in vitro. We find that LIF minimally influences the survival and differentiation of dentate gyrus neurons, causing a slight reduction of the number of dendrites per neuron. In contrast, LIF induces a pronounced increase in the number of astrocytes. This increase does not appear to be due to enhanced proliferation but rather to increased cell survival. On the other hand, epidermal growth factor (EGF) induces astrocyte proliferation, and addition of LIF inhibits the EGF effect. In summary, LIF does not appear to be crucial for the survival or differentiation of cultured dentate gyrus neurons. This cytokine increases astrocyte survival but does not enhance astrocyte proliferation, and LIF is able to counteract the growth stimulation elicited by EGF.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0006-8993(98)00236-4DOIArticle
http://www.sciencedirect.com/science/article/pii/S0006899398002364PublisherArticle
Additional Information:© 1998 Elsevier Science B.V. Accepted 24 February 1998. Available online 27 April 1999. We thank Lisa Banner for her comments on the manuscript. This work was supported by a NINDS grant to P.H.P, a NSF grant IBN 9319710. to D.H., a NSF research opportunity award to P.L. and fellowships from the Ciba-Geigy Jubilee Foundation, Sandoz Pharma, and the Foundation Theodor Engelmann to R.A.G.
Funders:
Funding AgencyGrant Number
National Institute of Neurological Disorders and Stroke (NINDS)UNSPECIFIED
NSFIBN 9319710
Ciba-Geigy Jubilee FoundationUNSPECIFIED
Sandoz PharmaUNSPECIFIED
Theodor Engelmann FoundationUNSPECIFIED
Subject Keywords:LIF; EGF; gp130; Hippocampus; Astrocyte
Issue or Number:1-2
Record Number:CaltechAUTHORS:20160317-081243333
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160317-081243333
Official Citation:Reto A Gadient, Pamela Lein, Dennis Higgins, Paul H Patterson, Effect of leukemia inhibitory factor (LIF) on the morphology and survival of cultured hippocampal neurons and glial cells, Brain Research, Volume 798, Issues 1–2, 6 July 1998, Pages 140-146, ISSN 0006-8993, http://dx.doi.org/10.1016/S0006-8993(98)00236-4. (http://www.sciencedirect.com/science/article/pii/S0006899398002364)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65411
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 Mar 2016 20:03
Last Modified:03 Oct 2019 09:47

Repository Staff Only: item control page