Gadient, Reto A. and Lein, Pamela and Higgins, Dennis and Patterson, Paul H. (1998) Effect of leukemia inhibitory factor (LIF) on the morphology and survival of cultured hippocampal neurons and glial cells. Brain Research, 798 (1-2). pp. 140-146. ISSN 0006-8993. doi:10.1016/S0006-8993(98)00236-4. https://resolver.caltech.edu/CaltechAUTHORS:20160317-081243333
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Abstract
Leukemia inhibitory factor (LIF) is a cytokine involved in the survival, differentiation and regeneration of sympathetic, sensory and motor neurons. Its effects in the brain are less well characterized. In a previous study, we found LIF transcripts to be predominantly expressed in neurons of the adult rat brain. Highest levels were observed in the hippocampus, particularly in granular neurons of the dentate gyrus and in hilar interneurons. Here we report the effects of LIF on survival and differentiation of postnatal rat hippocampal cells in vitro. We find that LIF minimally influences the survival and differentiation of dentate gyrus neurons, causing a slight reduction of the number of dendrites per neuron. In contrast, LIF induces a pronounced increase in the number of astrocytes. This increase does not appear to be due to enhanced proliferation but rather to increased cell survival. On the other hand, epidermal growth factor (EGF) induces astrocyte proliferation, and addition of LIF inhibits the EGF effect. In summary, LIF does not appear to be crucial for the survival or differentiation of cultured dentate gyrus neurons. This cytokine increases astrocyte survival but does not enhance astrocyte proliferation, and LIF is able to counteract the growth stimulation elicited by EGF.
Item Type: | Article | ||||||||||||
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Additional Information: | © 1998 Elsevier Science B.V. Accepted 24 February 1998. Available online 27 April 1999. We thank Lisa Banner for her comments on the manuscript. This work was supported by a NINDS grant to P.H.P, a NSF grant IBN 9319710. to D.H., a NSF research opportunity award to P.L. and fellowships from the Ciba-Geigy Jubilee Foundation, Sandoz Pharma, and the Foundation Theodor Engelmann to R.A.G. | ||||||||||||
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Subject Keywords: | LIF; EGF; gp130; Hippocampus; Astrocyte | ||||||||||||
Issue or Number: | 1-2 | ||||||||||||
DOI: | 10.1016/S0006-8993(98)00236-4 | ||||||||||||
Record Number: | CaltechAUTHORS:20160317-081243333 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160317-081243333 | ||||||||||||
Official Citation: | Reto A Gadient, Pamela Lein, Dennis Higgins, Paul H Patterson, Effect of leukemia inhibitory factor (LIF) on the morphology and survival of cultured hippocampal neurons and glial cells, Brain Research, Volume 798, Issues 1–2, 6 July 1998, Pages 140-146, ISSN 0006-8993, http://dx.doi.org/10.1016/S0006-8993(98)00236-4. (http://www.sciencedirect.com/science/article/pii/S0006899398002364) | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 65411 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Ruth Sustaita | ||||||||||||
Deposited On: | 17 Mar 2016 20:03 | ||||||||||||
Last Modified: | 10 Nov 2021 23:45 |
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