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Live confocal imaging of Arabidopsis flower buds

Prunet, Nathanaël and Jack, Thomas P. and Meyerowitz, Elliot M. (2016) Live confocal imaging of Arabidopsis flower buds. Developmental Biology, 419 (1). pp. 114-120. ISSN 0012-1606. PMCID PMC5025338. doi:10.1016/j.ydbio.2016.03.018. https://resolver.caltech.edu/CaltechAUTHORS:20160321-130327820

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Abstract

Recent advances in confocal microscopy, coupled with the development of numerous fluorescent reporters, provide us with a powerful tool to study the development of plants. Live confocal imaging has been used extensively to further our understanding of the mechanisms underlying the formation of roots, shoots and leaves. However, it has not been widely applied to flowers, partly because of specific challenges associated with the imaging of flower buds. Here, we describe how to prepare and grow shoot apices of Arabidopsis in vitro, to perform both single-point and time-lapse imaging of live, developing flower buds with either an upright or an inverted confocal microscope.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ydbio.2016.03.018DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025338PubMed CentralArticle
ORCID:
AuthorORCID
Prunet, Nathanaël0000-0002-8939-5920
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2016 Elsevier B.V. Received date: 1 February 2016; Revised date: 11 March 2016; Accepted date: 14 March 2016; Available online 15 March 2016. The authors wish to thank Ann Lavanway at Dartmouth and Andres Collazo at the Biological Imaging Facility at Caltech for their help in solving technical issues with the live confocal imaging. Funding in the Meyerowitz Laboratory was provided by the Howard Hughes Medical Institute, the US National Institutes of Health through grant R01 GM104244 and the Gordon and Betty Moore Foundation through Grant GBMF3406. Funding in the Jack lab was provided by the US National Science Foundation through grant IOS-0926347. Author contributions: NP did the imagery and wrote the manuscript. TPJ and EMM edited the manuscript.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHR01 GM104244
Gordon and Betty Moore Foundation3406
NSFIOS-0926347
Subject Keywords:Flower; flower development; flower meristem; plant development; confocal microscopy; live confocal imaging; sepals; floral organs
Issue or Number:1
PubMed Central ID:PMC5025338
DOI:10.1016/j.ydbio.2016.03.018
Record Number:CaltechAUTHORS:20160321-130327820
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160321-130327820
Official Citation:Nathanaël Prunet, Thomas P. Jack, Elliot M. Meyerowitz, Live confocal imaging of Arabidopsis flower buds, Developmental Biology, Volume 419, Issue 1, 1 November 2016, Pages 114-120, ISSN 0012-1606, http://dx.doi.org/10.1016/j.ydbio.2016.03.018. (http://www.sciencedirect.com/science/article/pii/S0012160616300665)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65538
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Mar 2016 01:01
Last Modified:10 Nov 2021 23:47

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