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Sindbis virus mutant ts20 of complementation group E contains a lesion in glycoprotein E2

Lindqvist, Björn H. and Disalvo, Jerry and Rice, Charles M. and Strauss, James H. and Strauss, Ellen G. (1986) Sindbis virus mutant ts20 of complementation group E contains a lesion in glycoprotein E2. Virology, 151 (1). pp. 10-20. ISSN 0042-6822. doi:10.1016/0042-6822(86)90099-1.

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A technique has been devised to readily obtain the entire structural protein region of Sindbis virus cloned into a plasmid vector. This method uses the fact that the nearest site for restriction enzyme HindIII to the 3’ terminal poly(A) occurs at nucleotides 6266-6271 in the genomic RNA. Inserts extending from the poly(A) tract to this HindIII site are 5438 nucleotides long (excluding the poly A tract) and contain the entire 4106-nucleotide structural protein region. Using an oligo(dT)-tailed vector as a primer for first strand cDNA synthesis such clones could be obtained in high yield. We were interested in a precise determination of the mutation responsible for the temperature-sensitive phenotype of ts20, a mutant belonging to complementation group E which has a defect in the function of glycoprotein E2 at the nonpermissive temperature. Using this technique we have cloned and sequenced the structural protein region of t-320 and of several revertants and concluded that the mutation was a change from histidine to leucine at amino acid 291 of E2. Reversion to temperature insensitivity occurred by same site reversion to the parental nucleotide, restoring the original histdine as amino acid 291. Thus, complementation group E of Sindbis virus results from changes in glycoprotein E2 and together with previous results from our laboratory (Arias et al, 1983; Hahn et al, 1985) demonstrates that the three RNA^+ complementation groups of Sindbis virus, C, D, and E, result from changes in the three structural proteins of the virus, capsid, glycoprotein El, and glycoprotein E2, respectively.

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Additional Information:Copyright © 1986 by Academic Press, Inc. All rights of reproduction in any form reserved. Received November 20, 1985; accepted January 24, 1986 We acknowledge the expert technical assistance of Ms. Edith Lenches who maintained the virus stocks and isolated the revertants studied here. One of us (B.H.L.) acknowledges a travel grant from the Norwegian Research Council for Science and Humanities in connection with a sabbatical stay at Caltech. This work was supported in part by Grants AI 10793 and AI 20612 from the National Institutes of Health and by Grant PCM 8316856 from the National Science Foundation, and by Biomedical Research Support Grant RR 07003.
Funding AgencyGrant Number
Norwegian Research Council for Science and HumanitiesUNSPECIFIED
NIHAI 10793
NIHAI 20612
NSFPCM 8316856
Biomedical Research Support GrantRR 07003
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Record Number:CaltechAUTHORS:20160321-150516795
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ID Code:65558
Deposited By: Donna Wrublewski
Deposited On:22 Mar 2016 19:36
Last Modified:10 Nov 2021 23:47

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