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Expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses

Hahn, Y. S. and Lenches, Edith M. and Galler, R. and Rice, C. M. and Dalrymple, J. and Strauss, J. H. (1990) Expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses. Archives of Virology, 115 (3-4). pp. 251-265. ISSN 0304-8608. doi:10.1007/BF01310534.

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Vaccinia virus recombinants were constructed which contained cDNA sequences encoding the structural region of dengue 2 virus (PR159/S1 strain) or yellow fever virus (17D strain). The flavivirus cDNA sequences were expressed under the control of the vaccinia 7.5k early/late promotor. Cultured cells infected with these recombinants expressed immunologically reactive flavivirus structural proteins, precursor prM and E. These proteins appeared to be cleaved and glycosylated properly since they comigrated with the authentic proteins from dengue 2 virus- and yellow fever virus-infected cells. Mice immunized with the dengue/vaccinia recombinant showed a dengue-specific immune response that included low levels of neutralizing antibodies. Immunization of mice with the yellow fever/vaccinia recombinant was less effective at inducing an immune response to yellow fever virus and in only some of the mice were low titers of neutralizing antibodies produced.

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Additional Information:Copyright (c) 1997 Institute for Scientific Information. Received March 9, 1990. Accepted June 11, 1990. We thank our colleagues for helpful suggestions on the manuscript and Dr. Chang Soo Hahn for help preparing the figures. This work was supported by a grant from the World Health Organization and U.S. Army Contract DAMD 17-87-C-7154. CMR is a Pew Scholar in the Biomedical Sciences. RG is a fellow from the National Research Council of Brazil (CNPq).
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World Health OrganizationUNSPECIFIED
U.S. ArmyDAMD 17-87-C-7154
Issue or Number:3-4
Record Number:CaltechAUTHORS:20160321-163102094
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65563
Deposited By: Donna Wrublewski
Deposited On:23 Mar 2016 23:22
Last Modified:10 Nov 2021 23:47

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