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Digit Induction by Hensen's Node and Notochord Involves the Expression of shh but Not RAR-β2

Selleck, Mark A. J. and Hayamizu, Terry F. and Ohsugi, Kojune and Bronner-Fraser, Marianne and Bryant, Susan V. (1996) Digit Induction by Hensen's Node and Notochord Involves the Expression of shh but Not RAR-β2. Developmental Biology, 173 (1). pp. 318-326. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20160322-094126495

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Abstract

It is well established that Hensen's nodes can induce the formation of supernumerary digits after grafting into the anterior margin of the developing limb bud. The recent finding that distinct mesodermal cell populations are segregated within the node has made it possible to isolate different prospective cell types in an attempt to correlate digit-inducing ability with cell fate. We find that the prospective notochord cells contained within Hensen's node are able to induce supernumerary digits, whereas presumptive somite cells cannot. This early difference in inducing ability persists into later stages of development: epithelial somites are unable to induce while notochord from all lengths of the neuraxis continues to induce. Using probes to retinoic acid receptor-β2 and sonic hedgehog (shh) we find no evidence to support the idea that inducing tissues generate extra digits by releasing retinoic acid into adjacent limb tissue but find that the inducing ability of a tissue correlates with its expression of shh.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.1996.0026DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160696900263PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1996 Academic Press, Inc. Received 14 June 1995, Accepted 31 October 1995, Available online 7 May 2002. We are grateful to Drs. David Gardiner and Mary Dickinson and the anonymous reviewers for helpful comments on the manuscript. We thank Bruce Blumberg and Kaz Umesono for the RAR-β2 probe and Juan Carlos Izpisua-Belmonte for the ssh probe. M.A.J.S. was supported by a Muscular Dystrophy Association research fellowship. Research was supported by HD25138 and a grant from March of Dimes Birth Defects Foundation to M.B.-F and HD25620 to S.V.B.
Funders:
Funding AgencyGrant Number
Muscular Dystrophy AssociationUNSPECIFIED
NIHHD25138
March of Dimes Birth Defects FoundationUNSPECIFIED
NIHHD25620
Issue or Number:1
Record Number:CaltechAUTHORS:20160322-094126495
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160322-094126495
Official Citation:Mark A.J. Selleck, Terry F. Hayamizu, Kojune Ohsugi, Marianne Bronner-Fraser, Susan V. Bryant, Digit Induction by Hensen's Node and Notochord Involves the Expression of shh but Not RAR-β2, Developmental Biology, Volume 173, Issue 1, 10 January 1996, Pages 318-326, ISSN 0012-1606, http://dx.doi.org/10.1006/dbio.1996.0026. (http://www.sciencedirect.com/science/article/pii/S0012160696900263)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:65584
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:22 Mar 2016 17:47
Last Modified:03 Oct 2019 09:48

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